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Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus

文献类型： 外文期刊

作者： Lu, Taofeng 1 ; Wang, Yuanzhi 2 ; Wu, Yanjun 1 ; Zhao, Lili 2 ; Wu, Shuguang 1 ; Chen, Hongyan 2 ;

作者机构： 1.Guizhou Univ Tradit Chinese Med, Inst Lab Anim Res, Guiyang 550025, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期：

页码：

收录情况： SCI

摘要： Aleutian mink disease (AMD), caused by Aleutian mink disease virus (AMDV), is a very important infectious disease of mink. Currently, elimination of antibody- or antigen-positive animals is the most successful strategy for eradicating AMD, but the claw-cutting method of blood sampling is difficult to perform and painful for the animal. In this study, we aimed to establish an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) method for the efficient detection of AMDV antigens using fecal samples. A purified mouse monoclonal antibody (mAb) was used as the capture antibody, and a rabbit polyclonal antibody (pAb) was used as the detection antibody. The assay was optimized by adjusting a series of parameters. Using a cutoff value of 0.205, the limit of detection of the AC-ELISA for strain AMDV-G antigen was 2 mu g/mL, and there was no cross-reaction with other mink viruses. The intra- and inter-assay standard deviations were below 0.046, and the correlation of variance (CV) values were 1.24-7.12% when testing fecal samples. Compared with conventional PCR results, the specificity and sensitivity were 91.5% and 90.6%, respectively, and the concordance rate between the two methods was 91.1%.

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