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Design of a universal primer pair for the identification of deer species

文献类型： 外文期刊

作者： Xie, Liuwei 1 ; Deng, Yongyan 2 ; Shao, Xiqun 1 ; Hu, Pengfei 2 ; Zhao, Dawei 2 ; Li, Chunyi 2 ; Ba, Hengxing 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Special Wild Econ Anim & Plants, 4899 Juye St, Changchun 130112, Peoples R China

2.Changchun Sci Tech Univ, Inst Antler Sci & Prod Technol, 1345 Pudong Rd, Changchun 130000, Peoples R China

关键词： Deer; Universal primer; Nuclear gene; CEP295NL; Diagnostic tool; Species identification

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

ISSN： 1877-7252

年卷期：

页码：

收录情况： SCI

摘要： Deer species has both scientific research and economic value, and half of these species, however, are listed as endangered animals. For the conservation purpose, we designed a novel universal deer-specific PCR primer pair based on an evolutionarily conservative coding sequence (i.e., CEP295NL gene) across some deer species. This primer pair was successfully amplified and sequenced, showing around similar to 540 bp in cervids. Validation results showed that it can be utilized to develop a reliable and simple diagnostic tool for distinguishing other closely related species, as well as possibly interspecific identification amongst cervids.

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