您好，欢迎访问四川省农业科学院 机构知识库！

Molecular cloning and expression analysis of EjSWEET15, enconding for a sugar transporter from loquat

文献类型： 外文期刊

作者： Li, Jing 1 ; Chen, Dong 1 ; Jiang, Guo-liang 1 ; Song, Hai-yan 1 ; Tu, Mei-yan 1 ; Sun, Shu-xia 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Hort Res Inst, Chengdu 610066, Sichuan, Peoples R China

2.Fruit Technol Promot Stn Longquanyi Dist, Chengdu 610100, Sichuan, Peoples R China

关键词： Loquat; SWEET; RACE; Sugar transport

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2020 年 272 卷

页码：

收录情况： SCI

摘要： Sugar transport is an essential physiological aspect with a crucial role in metabolism, growth and development in most living organisms. A specific class of protein (sugar transporter) is responsible for this sugar transportation task; with SWEET being one of the recently reported. Here, we used the PCR based rapid amplification of cDNA ends (RACE) technique to isolate a SWEET gene from fruits of a mutant while-flesh loquat designated as EjSWEET15. Our findings based on qRT-PCR revealed that EjSWEET15 was highly expressed in leaves and fruits. Interestingly, this expression was at a significant higher level in fruits of mutant while-flesh loquat as to that of the wild-type red-flesh loquat counterpart. EjSWEET15 presents 4 polymorphic loci in mutant of while-flesh loquat attributing to amino acid changes with one located in the promoter region and three within the open reading frame as disclosed by nucleotide polymorphism analysis. It is speculated that these differential gene expression and sequence modification may affect the enzymatic activity of EjSWEET15 and thus letting EjSWEET15to play an important role in the regulation of sugar transport in loquat.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台