文献类型： 外文期刊

作者： Tai, Wanbo ¹ ; Tian, Chongyu ¹ ; Shi, Huicheng ² ; Chai, Benjie ² ; Yu, Xinyang ⁴ ; Zhuang, Xinyu ⁵ ; Dong, Pengyuan ¹ ; Li, Min ⁶ ; Yin, Qi ⁶ ; Feng, Shengyong ² ; Wang, Weixiao ¹ ; Zhang, Oujia ² ; Liang, Shibo ¹ ; Liu, Yang ¹ ; Liu, Jianying ¹ ; Zhu, Longchao ¹ ; Zhao, Guangyu ⁶ ; Tian, Mingyao ⁵ ; Yu, Guocan ⁴ ; Cheng, Gong ² ;

作者机构： 1.Shenzhen Bay Lab, Inst Infect Dis, Shenzhen, Peoples R China

2.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Sch Med, New Cornerstone Sci Lab, Beijing, Peoples R China

3.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing, Peoples R China

4.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing, Peoples R China

5.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China

6.Acad Mil Med Sci, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China

7.Shenzhen Ctr Dis Control & Prevent, Inst Pathogen Organisms, Shenzhen, Peoples R China

8.Southwest United Grad Sch, Kunming, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：15.7； 五年影响因子：17.2 ）

ISSN：

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： The persistent monkeypox outbreaks intensify the demand for monkeypox vaccines. Based on the mRNA vaccine platform, we conduct a systematic screening of monkeypox virus (MPXV) surface proteins from two types of viral particles, extracellular enveloped viruses (EVs) and intracellular mature viruses (MVs). This screening unveils 12 important antigens with diverse levels of neutralizing immunogenicity. Further assessment reveals that the combinations of 4, 8, and 12 of these antigens, namely Mix-4, Mix-8, and Mix-12, induce varying degrees of immune protection, with Mix-12 being the most potent. This finding demonstrates the significance of not only the level but also the diversity of the neutralizing antibodies in providing potent immune protection. Additionally, we utilize a T cell-epitope enrichment strategy, analyzing the complete proteome sequence of the MPXV to predict antigenic epitope-rich regions. Integration of these epitope-rich regions into a cellular immune-targeting antigen, named MPX-EPs, showcases that a cellular immune-targeting mRNA vaccine can independently confer immune protection. Furthermore, co-immunization with Mix-12 and MPX-EPs achieves complete protection against MPXV challenge. Overall, these results suggest an effective approach to enhance the immune protection of mRNA vaccines through the specific coordination of humoral and cellular immune responses.