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Comparison and evaluation of DNA vaccines against Nocardia seriolae infection in largemouth bass ( Micropterus salmoides)

文献类型： 外文期刊

作者： Wang, Dandan 1 ; Zhang, Bo 1 ; Chen, Ming 1 ; Zeng, Hui 1 ; Zhang, Xu-Jie 1 ; Zhang, Yong-An 1 ; Zhou, Yang 1 ;

作者机构： 1.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Coll Fisheries, Natl Key Lab Agr Microbiol,Hubei Hongshan Lab,Engn, Wuhan, Peoples R China

2.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China

3.Chinese Acad Agr Sci, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab, Shenzhen Branch,Agr Genom Inst Shenzhen,Minist Agr, Shenzhen, Peoples R China

关键词： Largemouth bass; Nocardia seriolae; DNA vaccine

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 596 卷

页码：

收录情况： SCI

摘要： Chronic granulomatous diseases caused by Nocardia seriolae have resulted in substantial economic losses for the aquaculture industry. DNA vaccines are considered a promising approach for controlling infectious diseases. To develop effective DNA vaccines against N. seriolae, we selected Ag85L, MmaA4, HspX, Rv3407, and HtpG as candidate antigens. Among these, pcDNA-Ag85L and pcDNA-MmaA4 were the most effective against N. seriolae infection in largemouth bass (Micropterus salmoides), achieving the highest relative percentage survival (RPS) rates of 78.6 % and 92.9 %, respectively. Tissue bacterial loads and histopathological tests revealed a significant reduction in bacterial abundance in the liver, spleen, head kidney, and trunk kidney of the immunised groups compared to that in the control group. Additionally, no distinct pathological changes were observed in the spleen or head kidney tissues. The expression levels of immune-related genes including IL-1 beta, IL-8, MHCI, MHCII, CD4, and CD8 alpha were significantly upregulated in the pcDNA-Ag85L and pcDNA-MmaA4 immunised groups, thus indicating that the two DNA vaccines mediated immune protection by promoting both humoral and cellular immune responses. Overall, the pcDNA-Ag85L and pcDNA-MmaA4 DNA vaccines demonstrated strong potential for protecting largemouth bass against N. seriolae infection, offering an innovative solution for controlling granulomatous diseases in fish.

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