您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Optimizing the zymogram of exogenous proteases in broiler diets by in vitro simulated gastrointestinal digestion and response surface methodology

文献类型： 外文期刊

作者： Liu, Yang 1 ; Liu, Shijie 3 ; Chen, Fengming 1 ; Liu, Shengli 4 ; Lin, Qian 1 ; Dai, Qiuzhong 2 ;

作者机构： 1.Changsha Med Univ, Hunan Prov Key Lab Tradit Chinese Med Agr Biogenom, Changsha 410219, Peoples R China

2.Hunan Inst Anim Husb & Vet Med, Dept Anim Nutr, Changsha 410125, Peoples R China

3.Univ Sheffield, Dept Chem & Biol Engn, Sheffield S13JD, England

4.Shandong Lonct Enzymes Co Ltd, Linyi 276400, Peoples R China

5.Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China

关键词： In vitro simulated digestion; Poultry diet; Protease; Response surface methodology; Zymogram

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Low protein digestibility causes intestinal flora imbalance, wet litter, and nitrogen pollution. The present study aimed to explore the optimal zymogram of exogenous acid protease (ACP), neutral protease (NEP), alkaline protease (ALP), and keratinase (KEA) in corn-soybean based diets for broilers. The hydrolysis performances of the four monocomponent proteases were presented by enzymatic hydrolysate gross energy (EHGE) and improved dry matter digestibility (IDMD), which were tested via the in vitro simulated digestion method. The optimal combination of the four proteases was predicted by the response surface method. Results showed that the optimal zymogram for 1 to 3-wk-old broiler diet was 1.80 U/g ACP, 2.30 U/g NEP, 29.30 U/g ALP, and 2.80 U/g KEA, and the EHGE and IDMD reached 94.65 Cal/g and 2.54%. The optimal zymogram for 4 to 6-wk-old broiler diet was 1.50 U/g ACP, 1.90 U/g NEP, 31.53 U/g ALP, and 3.10 U/g KEA, and the EHGE and IDMD reached 92.29 Cal/g and 2.47%. The performances of the predicted optimal zymogram were further verified by in vitro simulated digestion method. Collectively, the combined use of four proteases could improve the protein digestibility in broiler diets, which had better effect than monocomponent protease.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台