Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research
文献类型： 外文期刊
作者： Wang, Shuo 1 ; Blume, Rostislav Y. 2 ; Zhou, Zhi-Wei 1 ; Lu, Shaoping 1 ; Nazarenus, Tara J. 3 ; Blume, Yaroslav B. 2 ; Xie, Weibo 1 ; Cahoon, Edgar B. 3 ; Chen, Ling-Ling 5 ; Guo, Liang 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
2.Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine
3.Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA
4.Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA
5.Guangxi Univ, Coll Life Sci & Technol, Nanning, Peoples R China
6.Hubei Hongshan Lab, Wuhan, Peoples R China
7.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan, Peoples R China
8.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,G, Shenzhen, Peoples R China
关键词： Camelina neglecta; Camelina sativa; Synteny analysis; Camelina evolution; Oil biosynthesis pathway; Medium-chain fatty acid
期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
ISSN：
年卷期： 2024 年 17 卷 1 期
页码：
收录情况： SCI
摘要： Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop Camelina sativa. Here, we report a chromosome-level assembly of C. neglecta with a total length of 210 Mb. By adopting PacBio sequencing and Hi-C technology, the C. neglecta genome was assembled into 6 chromosomes with scaffold N50 of 29.62 Mb. C. neglecta has undergone the whole-genome triplication (gamma) shared among eudicots and two whole-genome duplications (alpha and beta) shared by crucifers, but it has not undergone a specific whole-genome duplication event. By synteny analysis between C. neglecta and C. sativa, we successfully used the method of calculating Ks to distinguish the three subgenomes of C. sativa and determined that C. neglecta was closest to the first subgenome (SG1) of C. sativa. Further, transcriptomic analysis revealed the key genes associated with seed oil biosynthesis and its transcriptional regulation, including SAD, FAD2, FAD3, FAE1, ABI3, WRI1 and FUS3 displaying high expression levels in C. neglecta seeds. The high representability of C. neglecta as a model species for Camelina-based biotechnology research has been demonstrated for the first time. In particular, floral Agrobacterium tumefaciens infiltration-based transformation of C. neglecta, leading to overexpression of CvLPAT2, CpDGAT1 and CvFatB1 transgenes, was demonstrated for medium-chain fatty acid accumulation in C. neglecta seed oil. This study provides an important genomic resource and establishes C. neglecta as a new model for oilseed biotechnology research.
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