您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters

文献类型： 外文期刊

作者： Liu, Mengqian 1 ; Tan, Lei 1 ; Li, Xinlu 1 ; Li, Haojie 1 ; Zhang, Yong 1 ; Zi, Xiannian 1 ; Ge, Changrong 1 ; Wang, Kun 1 ;

作者机构： 1.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China

2.Chinese Acad Agr Sci, Inst Feed Res, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China

3.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Gansu, Peoples R China

关键词： Transcriptome; Metabolome; light intensity; Chahua No.2 rooster; Testosterone

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 4 期

页码：

收录情况： SCI

摘要： This study investigated the impact of light duration on Testosterone secretion in Chahua No.2 roosters, utilizing combined Transcriptome and Metabolome analyses to uncover critical genes, Metabolites, and signaling pathways. We randomly selected 240 Chahua No.2 roosters at 42 days old and divided them into four groups: simulated natural light (I), 12L:12D (II), 16L:8D (III), and 20L:4D (IV), each group has 6 replicates, with 10 chickens per replicate. Blood samples were collected at 91 and 140 days post-hatch to measure Testosterone levels. Results showed that at 91 days, group IV had significantly higher Testosterone levels than groups I, II, and III (P G 0.01), with group III also higher than groups I (P G 0.01) and II (P G 0.05). By 140 days, group IV maintained significantly higher Testosterone than groups I and II (P G 0.01) and higher than group III (P G 0.05), while group III was also elevated compared to groups I and II (P G 0.05). Testicles Transcriptomics analysis revealed 891 differentially expressed genes, including 479 down-regulated and 412 up-regulated genes. Key signaling pathways identified included Steroid Hormone Biosynthesis, Cytochrome P450, and retinol metabolism. Testicles Metabolomics analysis identified 174 differential Metabolites, with 91 up-regulated and 83 down-regulated, focusing on pathways like Amino Sugar Metabolism and Tryptophan Metabolism. Integrated analysis pinpointed 19 common signaling pathways, with the top ten including Cytochrome P450, tyrosine metabolism, and amino acid biosynthesis. Our findings indicate that extending light duration enhances Testosterone secretion in roosters. Through comprehensive transcriptomic and metabolomic analyses, we established that pathways associated with steroid hormone synthesis and Cytochrome P450 play a crucial role in light duration-regulated Testosterone secretion, highlighting key genes such as CYP11A1, CYP17A1, and HSD3B1, alongside Metabolites like ergosterol-5,7,22,24(28)-tetraene-3beta-alcohol.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台