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Quantitative trait locus mapping and identification of candidate genes for resistance to Verticillium wilt in four recombinant inbred line populations of Gossypium hirsutum

文献类型： 外文期刊

作者： Wang, Yuxiang 1 ; Zhao, Jieyin 1 ; Chen, Qin 1 ; Zheng, Kai 1 ; Deng, Xiaojuan 1 ; Gao, Wenju 1 ; Pei, Wenfeng 3 ; Geng, Shiwei 1 ; Deng, Yahui 1 ; Li, Chunping 2 ; Chen, Quanjia 1 ; Qu, Yanying 1 ;

作者机构： 1.Xinjiang Agr Univ, Coll Agr, Engn Res Ctr Cotton, Minist Educ, 311 Nongda East Rd, Urumqi 830052, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Cash Crops, Urumqi 830052, Peoples R China

3.Chinese Acad Agr Sci, Minist Agr, State Key Lab Cotton Biol, Key Lab Cotton Genet Improvement,Inst Cotton Res, Anyang, Peoples R China

关键词： Gossypium hirsutum; Verticillium wilt; QTL; Gh_CPR30

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2023 年 327 卷

页码：

收录情况： SCI

摘要： Improving resistance to Verticillium wilt is of great significance for achieving high and stable yields of Upland cotton (Gossypium hirsutum). To deeply understand the genetic basis of cotton resistance to Verticillium wilt, Verticillium wilt-resistant Upland Lumianyan 28 and four Verticillium wilt-susceptible Acala cotton cultivars were used to create four recombinant inbred line (RIL) populations of 469 families through nested hybridization. Phenotypic data collected in five stressful environments were used to select resistant and sensitive lines and create a mixed pool of extreme phenotypes for BSA-seq. A total of 8 QTLs associated with Verticillium wilt resistance were identified on 4 chromosomes, of which qVW-A12-5 was detected simultaneously in the RIL populations and in one of the RIL populations and was identified for the first time. According to the sequence comparison and transcriptome analysis of candidate genes in the QTL interval between parents and pools, 4 genes were identified in the qVW-A12-5 interval. qRT-PCR of parental and phenotypically extreme lines revealed that Gh_CPR30 was induced by and may be a candidate gene for resistance to Verticillium wilt in G. hirsutum. Furthermore, VIGS technology revealed that the disease severity index (DSI) of the Gh_CPR30silenced plants was significantly higher than that of the control. These results indicate that the Gh_CPR30 gene plays an important role in the resistance of G. hirsutum to Verticillium wilt, and the study provides a molecular basis for analyzing the molecular mechanism underlying G. hirsutum resistance to Verticillium wilt.

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