Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90
文献类型： 外文期刊
作者： Wang, Yingwen 1 ; Qi, Peng 1 ; Zhang, Dun 1 ; Zeng, Yan 1 ; Sun, Yan 1 ; Wang, Peng 1 ;
作者机构： 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Chinese Acad Agr Sci, Key Lab Tobacco Pest Monitoring & Integrated Manag, Tobacco Res Inst, Qingdao 266101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
关键词： Paper-based ATP assay; Degradation of high viscosity hydrogel; Nanozyme reactivation; Onsite detection; ZIF-90
期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：15.1； 五年影响因子：14.3 ）
ISSN： 1385-8947
年卷期： 2024 年 481 卷
页码：
收录情况： SCI
摘要： Adenosine triphosphate (ATP) is an important biological biomarker, but its rapid extracellular decomposition poses challenges for on-site detection. We report a paper-based dual-readout viscosity flow sensor enabling rapid, equipment-free quantification of ATP. The assay integrates molybdenum disulfide nanozymes for signal amplification with zeolitic imidazolate framework-90 (ZIF-90) for selective ATP recognition and nanozyme regulation. In the presence of ATP, the intact chitosanase-encapsulated ZIF-90 structure was disintegrated to release chitosanase and thus hydrolyzed chitosan hydrogel, decreasing the viscosity of chitosan solution. Moreover, elimination of ZIF-90 inhibitory effect reactivated the catalytic activity of MoS2 nanozyme, resulting in the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to produce obvious color change. The analytical signals, combining the distance- and colorimetric- readout, were directly displayed on filter paper without further processed. The proposed dual-readout viscosity flow sensor exhibited remarkable sensitivity for ATP detection with detection limits down to 0.038 nM with the distance-readout and 0.057 nM for the colorimetric-readout. Furthermore, we developed an integrated paper kit with a printed scale and color card for standardized on-site analysis without instrumentation. Successful application for ATP quantification in bacterial biofilms and blood samples demonstrates the sensor's strong performance and potential for decentralized testing.
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