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Utilization of Transcriptome, Small RNA, and Degradome Sequencing to Provide Insights Into Drought Stress and Rewatering Treatment in Medicago ruthenica

文献类型： 外文期刊

作者： Shi, Rui 1 ; Jiao, Wei 3 ; Yun, Lan 1 ; Zhang, Zhiqiang 1 ; Zhang, Xiujuan 5 ; Wang, Quanzhen 5 ; Li, Ying 1 ; Mi, Fugui 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Key Lab Grassland Resources, Minist Educ PR China, Coll Grassland Resources & Environm, Hohhot, Peoples R China

2.Baotou Med Coll, Baotou, Peoples R China

3.Inner Mongolia Agr Univ, Coll Mech & Elect Engn, Hohhot, Peoples R China

4.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China

5.Inner Mongolia Key Lab Mol Biol Featured Plants, Hohhot, Peoples R China

6.Northwest A&F Univ, Coll Grassland Agr, Yangling, Shaanxi, Peoples R China

关键词： Medicago ruthenica; drought; differential expression; miRNA; degradome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Drought is a major limiting factor in foraging grass yield and quality. Medicago ruthenica (M. ruthenica) is a high-quality forage legume with drought resistance, cold tolerance, and strong adaptability. In this study, we integrated transcriptome, small RNA, and degradome sequencing in identifying drought response genes, microRNAs (miRNAs), and key miRNA-target pairs in M. ruthenica under drought and rewatering treatment conditions. A total of 3,905 genes and 50 miRNAs (45 conserved and 5 novel miRNAs) were significantly differentially expressed in three test conditions (CK: control, DS: plants under drought stress, and RW: plants rewatering after drought stress). The degradome sequencing (AllenScore < 4) analysis revealed that 104 miRNAs (11 novel and 93 conserved miRNAs) were identified with 263 target transcripts, forming 296 miRNAtarget pairs in three libraries. There were 38 differentially expressed targets from 16 miRNAs in DS vs. CK, 31 from 11 miRNAs in DS vs. RW, and 6 from 3 miRNAs in RW vs. CK; 21, 18, and 3 miRNA-target gene pairs showed reverse expression patterns in DS vs. CK, DS vs. RW, and RW vs. CK comparison groups, respectively. These findings provide valuable information for further functional characterization of genes and miRNAs in response to abiotic stress, in general, and drought stress in M. ruthenica, and potentially contribute to drought resistance breeding of forage in the future.

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[1]Utilization of Transcriptome, Small RNA, and Degradome Sequencing to Provide Insights Into Drought Stress and Rewatering Treatment in Medicago ruthenica. Shi, Rui,Yun, Lan,Zhang, Zhiqiang,Li, Ying,Mi, Fugui,Shi, Rui,Jiao, Wei,Jiao, Wei,Zhang, Xiujuan,Wang, Quanzhen. 2021

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