文献类型： 外文期刊

作者： Li Feng-qi ² ; Fu Ning-ning ¹ ; Zhou Jing-jiang ³ ; Wang Gui-rong ² ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

3.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England

关键词： plant defence;ferpene synthases;(E)-beta-caryophyllene;lima bean

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 10 期

页码：

收录情况： SCI

摘要： (E)-beta-Caryophyllene is a sesquiterpene compound widely distributed in plants and functions in plant defence. However, little is known about the sequence and function of (E)-beta-caryophyllene synthase in lima bean (Phaseolus lunatus). Here, we report a new full-length cDNA (PICAHS) encoding (E)-beta-caryophyllene synthase, a possible key enzyme of plant defence. The cDNA of PICAHS contains an open reading frame of 1761 bp, encoding a protein of 586 amino acids with a predicted mass of 67.95 kDa. The deduced amino acid sequence shows 52% identity with sesquiterpene synthase MtCAHS of Medicago truncatula. Based on phylogenetic analysis, PICAHS is classified as the terpene synthases (TPS)-a subfamily. The recombinant enzyme, expressed in Escherichia coli, catalysed the formation of a major product (E)-beta-caryophyllene (82%) and a minor product a-humulene (18%) from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis found that the PICAHS transcript was significantly up-regulated in leaves after treatment with spider mites and alamethicin (ALA), suggesting its ecological function in plant defence.