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Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro)

文献类型: 外文期刊

作者: Yang, S. 1 ; Van Poucke, C. 4 ; Wang, Z. 3 ; Zhang, S. 3 ; De Saeger, S. 2 ; De Boevre, M. 2 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China

2.Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, Ottergemsesteenweg 460, B-9000 Ghent, Belgium

3.China Agr Univ, Beijing Lab Food Qual & Safety, Coll Vet Med, Beijing Lab Detect Technol Anim Derived Food Safe, Beijing 100193, Peoples R China

4.Flanders Res Inst Agr Fisheries & Food, Brusselsesteenweg 370, B-9090 Melle, Belgium

关键词: T-2 toxin;T-2 toxin-3-glucoside;metabolism;liver;UHPLC-QTOF

期刊名称:WORLD MYCOTOXIN JOURNAL ( 影响因子:3.353; 五年影响因子:3.108 )

ISSN: 1875-0710

年卷期: 2017 年 10 卷 4 期

页码:

收录情况: SCI

摘要: The metabolic profile of T-2 toxin-3-glucoside (T2-Glc) in humans and rats was investigated using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). When rat and human liver microsomes were incubated with T2-Glc, a total of five metabolites were detected. T2-Glc exposed a higher metabolic stability in rats and human than T-2 toxin (T-2). The metabolism of T2-Glc by the intestinal microbiota of human and rats was also investigated, and three metabolites were observed. T2-Glc was reconverted to T-2 during incubation with fresh faeces. Furthermore, in vivo metabolism of T2-Glc in rats after oral administration was carried out, and three metabolites were detected in rat urine and faeces (T-2, HT-2 toxin and 3'-OH-T2-Glc). In vivo metabolism results indicated that T2-Glc was mainly metabolised in the gastro-intestinal tract with a low absorption level in rats. The results demonstrated that hydroxylation (C-3' and C-4'), hydrolysis (C-4 and C-8) and deconjugation are the main metabolic pathways of T2-Glc in mammals.

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