您好,欢迎访问中国农业科学院 机构知识库!

Chicken model for inducing primordial germ cells differentiating into motile sperms in vitro

文献类型: 外文期刊

作者: Xiong, Hui 1 ; Li, Xiangchen 2 ; Hu, Qingyun 3 ; Hu, Pengfei 2 ; Guan, Weijun 2 ;

作者机构: 1.Jining Med Univ, Coll Basic Med, Histol & Embryol Dept, Jining 272000, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

3.Univ Jiamusi, Coll Basic Med, Jiamusi 154007, Peoples R China

关键词: chicken;primordial germ cells;retinoic acid;induction;motile sperm

期刊名称:AVIAN BIOLOGY RESEARCH ( 影响因子:1.224; 五年影响因子:1.061 )

ISSN: 1758-1559

年卷期: 2017 年 10 卷 1 期

页码:

收录情况: SCI

摘要: Stem cell technologies have been widely used in the study of spermatogenesis. However, deriving motile sperm from stem cells in vitro is still rarely achieved. We found that chicken primordial germ cells could directly differentiate into sperm by using retinoic acid in a non-testicular culture system. The induced sperms were characterised by RT-PCR, immunofluorescence and flow cytometry techniques. Results suggested that chicken primordial germ cells could produce motile sperm in vitro. Our work has provided a novel animal model of spermatogenesis in vitro, which might be used for male reproductive mechanism research.

  • 相关文献

[1]Cultivation and Biological Characterization of Chicken Primordial Germ Cells. Ji, Meng,Guan, Wei-jun,Gao, Yu-hua,Bai, Chun-yu,Ma, Yue-hui,Li, Xiang-chen,Li, Lu. 2016

[2]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[3]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[4]噬菌体展示技术分析鸡贫血病毒VP1蛋白抗原结构特征. 王晓艳,高玉龙,高宏雷,付朝阳,邓小芸,林欢,祁小乐,王笑梅. 2007

[5]Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip. Li, Jingchun,Ning, Bolin,Luo, Yinghua,Guo, Li,Wei, Guosheng,Liu, Shengjun,Zhang, Ying,Zhang, Aizhong,Wu, Rui,Li, Yanbing,Cao, Xinyan.

[6]Directed differentiation of chick embryonic germ cells into neural cells using retinoic acid induction in vitro. Du, Li-xin,Wang, Juan,Pan, Xiao-hong,Niu, Xin-hua,Jiao, Fei,Xie, Shu-yang,Li, Zun-ling. 2009

[7]Rapid detection of nandrolone residues in chicken by surface-enhanced Raman spectroscopy. Ning Xu,Muhua Liu,Haichao Yuan,Jinhui Zhao,Jinjiang Tao,Hongqing Guo,Jian Chen. 2018

[8]Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts. Dong, Xiao,Mu, Yulian,Liu, Lixin,Chen, Hongping,Zhang, Li,Feng, Shutang,Dong, Xiao,Tsung, Hsiaochien,Mu, Yulian,Feng, Shutang,Wang, Hongjun. 2014

[9]Embryoid bodies formation from chicken primordial germ cells. Xiong, Hui,Pu, Yabin,Shan, Zhiqiang,Hu, Pengfei,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[10]bFGF signaling-mediated reprogramming of porcine primordial germ cells. Zhang, Yu,Ma, Jing,Lv, Jiawei,Wei, Renyue,Cong, Yimei,Liu, Zhonghua,Li, Hai,Cong, Yimei.

[11]Effects of constant temperature, exposure period, and age on diapause induction in Trichogramma dendrolimi. Ma, CS,Chen, YW. 2006

[12]Effect of mouse calcineurin on induction and growth of rice callus transformed by the calcineurin gene. Ma, Xujun,Qian, Qian,Yang, Chuanping,Liu, Guifeng,Zhu, Dahai. 2006

[13]Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display. Cui, XL,Nagesha, HS,Holmes, IH.

[14]Functional analysis and induction of four novel goose (Anser cygnoides) avian beta-defensins in response to salmonella enteritidis infection. Ma, Deying,Zhou, Caiyuan,Zhang, Mingyue,Zhou, Caiyuan,Zhang, Mingyue,Han, Zongxi,Shao, Yuhao,Liu, Shengwang.

[15]In situ expression of the GmNMH7 gene is photoperiod-dependent in a unique soybean (Glycine max [L.] Merr.) flowering reversion system. Wu, CX,Ma, QB,Yam, KM,Cheung, MY,Xu, YY,Han, TF,Lam, HM,Chong, K.

[16]The transport of C-14-salicylic acid in heat-stressed young Vitis vinifera plants. Wang, LJ,Huang, WD,Zhan, JC,Yu, FY.

[17]Differentiation potential of bone marrow mesenchymal stem cells in duck. Li, Linfeng,Gong, Xuelian,Liu, Hongkun,Chen, Lina,Guan, Weijun,Ma, Yuehui,Li, Linfeng,Bai, Xiujuan. 2009

[18]Three novel Anas platyrhynchos avian beta-defensins, upregulated by duck hepatitis virus, with antibacterial and antiviral activities. Lin, Lijuan,Zhang, Kexin,Han, Zongxi,Shao, Yuhao,Liu, Xiaoli,Liu, Shengwang,Ma, Deying,Lin, Lijuan,Zhang, Kexin. 2011

[19]Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum). He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang,Li, Yi-Na,Zhang, Zhi-Fang,Su, Zhong-Liang,Shao, Hong-Bo.

[20]Determination of virginiamycin M1 residue in tissues of swine and chicken by ultra-performance liquid chromatography tandem mass spectrometry. Wang, Xiaoyang,Wang, Mi,Zhang, Keyu,Hou, Ting,Zhang, Lifang,Fei, Chenzong,Xue, Feiqun,Wang, Xiaoyang,Hang, Taijun. 2018

作者其他论文 更多>>