Chicken model for inducing primordial germ cells differentiating into motile sperms in vitro
文献类型: 外文期刊
作者: Xiong, Hui 1 ; Li, Xiangchen 2 ; Hu, Qingyun 3 ; Hu, Pengfei 2 ; Guan, Weijun 2 ;
作者机构: 1.Jining Med Univ, Coll Basic Med, Histol & Embryol Dept, Jining 272000, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
3.Univ Jiamusi, Coll Basic Med, Jiamusi 154007, Peoples R China
关键词: chicken;primordial germ cells;retinoic acid;induction;motile sperm
期刊名称:AVIAN BIOLOGY RESEARCH ( 影响因子:1.224; 五年影响因子:1.061 )
ISSN: 1758-1559
年卷期: 2017 年 10 卷 1 期
页码:
收录情况: SCI
摘要: Stem cell technologies have been widely used in the study of spermatogenesis. However, deriving motile sperm from stem cells in vitro is still rarely achieved. We found that chicken primordial germ cells could directly differentiate into sperm by using retinoic acid in a non-testicular culture system. The induced sperms were characterised by RT-PCR, immunofluorescence and flow cytometry techniques. Results suggested that chicken primordial germ cells could produce motile sperm in vitro. Our work has provided a novel animal model of spermatogenesis in vitro, which might be used for male reproductive mechanism research.
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