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Effect of iron source on iron absorption and gene expression of iron transporters in the ligated duodenal loops of broilers

文献类型: 外文期刊

作者: Zhang, L. Y. 1 ; Li, X. F. 1 ; Liao, X. D. 1 ; Zhang, L. Y. 1 ; Lu, L. 1 ; Luo, X. G. 1 ;

作者机构: 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Mineral Nutr Res Div, Beijing 100193, Peoples R China

2.Ulanqab Vocat Coll, Ulanqab 012000, Inner Mongolia, Peoples R China

3.Beijing ZKRZ AGR SCI TECHCL Co Ltd, Beijing 100085, Peoples R China

关键词: broiler;divalent metal transporter 1;ferroportin 1;iron absorption kinetics;organic iron source

期刊名称:JOURNAL OF ANIMAL SCIENCE ( 影响因子:3.159; 五年影响因子:2.912 )

ISSN: 0021-8812

年卷期: 2017 年 95 卷 4 期

页码:

收录情况: SCI

摘要: This experiment was conducted to investigate the effect of iron source on Fe absorption and the gene expression of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1) in the ligated duodenal loops of broilers. The in situ ligated duodenal loops from Fe-deficient broiler chicks (28-d-old) were perfused with Fe solutions containing 0 to 14.33 mmol Fe/L from 1 of the following: Fe sulfate (FeSO4 center dot 7H(2)O), Fe methionine with weak chelation strength (Fe-Met W; chelation strength is expressed as quotient of formation [Q(f)] value, Q(f) = 1.37), Fe proteinate with moderate chelation strength (Fe-Prot M; Q(f) = 43.6), and Fe proteinate with extremely strong chelation strength (Fe-Prot ES; Q(f) = 8,590) for up to 30 min. The gene expression of DMT1 and FPN1 in the duodenal loops from the control group and the groups treated with 3.58 mmol Fe/L from 1 of 4 Fe sources was analyzed. The absorption kinetics of Fe from different Fe sources in the duodenum followed a saturated carrier-dependent transport process. The maximum transport rate (Jmax) values in the duodenum were greater (P < 0.03) for Fe-Prot ES and Fe-Prot M than for Fe-Met W and FeSO4 center dot 7H(2)O. The Fe perfusion inhibited (P < 0.05) the mRNA expression of DMT1 but enhanced (P < 0.0008) the mRNA expression of FPN1 in the duodenum and had no effect (P > 0.14) on the protein expression levels of the 2 transporters. These results indicated that organic Fe sources with greater Q(f) values showed higher Fe absorption; however, all Fe sources followed the same saturated carrier-dependent transport process in the duodenum, and DMT1 and FPN1 might participate in Fe absorption in the duodenum of broilers regardless of Fe source.

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