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Molecular cloning and characterization of cotton cDNAs expressed in developing fiber cells

文献类型： 外文期刊

作者： Zhao, GR 1 ; Liu, JY 1 ; Du, XM 1 ;

作者机构： 1.Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455112, Henan, China

关键词： cDNA sequence;developing fiber cells;differential gene expression;Gossypium hirsutum

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

ISSN： 0916-8451

年卷期： 2001 年 65 卷 12 期

页码：

收录情况： SCI

摘要： Using the FDD-PCR technique, ten new fiber-specific cDNAs were isolated from developing cotton fiber cells and showed high amino acid identity to previously recorded cDNAs. Five cDNAs encoding bisphosphate nucleotidase, alpha -tubulin, beta -galactosidase, annexin, and reversibly glycosylated polypeptide were identified while the functions of five other cDNAs were undetermined. Dot blot analysis showed that all transcripts of the 10 cDNAs accumulated preferentially in fiber cells and the majority were expressed in the early phase of cotton fiber development, except for F14 which accumulated at a high level during the late phase of developing fiber cells, indicating that all of the genes were involved in the process of fiber development.

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