文献类型： 外文期刊
作者： Zhao Jin-Hong 1 ; Du Wei-Hua 2 ; Dao Er-Ji 2 ; Pei Jie 2 ; Zhang Li 2 ; Lin Xiu-Kun 3 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China; Inner Mongolia Agr Univ, Coll Anim Sci & Med, Hohhot 010018, Peoples R China; China NW Sci Tech Univ Agr & Forestry, Yangling 712100, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China;
关键词： siRNA;Sry;mouse embryos;gene silencing
期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）
ISSN： 1000-3282
年卷期： 2006 年 33 卷 9 期
页码：
收录情况： SCI
摘要： In order to study the regulation mechanism of Sry in sex determination, the siRNA technique was used to silence the. Sty expression in mouse embryos. Two siRNA expression vectors were synthesized, pSilencer4.1/Sry217 and pSilencer 4.1/Sry565. The siRNA expression vectors were injected into gestated mouse through tail vein. The sex was identified by PCR, and the expression level of Sry gene was determined using RT-PCR technique in the male mouse embryos at 11.5 dpc(days post coitum). The time- and dose -dependent effects was studied. The results confirmed that one of the siRNA expression plasmids, pSilencer4.1/Sry565, could inhibit the Sry gene expression significantly. Eighty-five percent inhibition was obtained when injecting 20 mu g siRNA vector at 9.5 dpc.
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