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The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B-juncea to Siclerotinia sclerotiorum

文献类型： 外文期刊

作者： Li, C. X. 1 ; Li, Hua 2 ; Siddique, A. B. 1 ; Sivasithamparam, K. 2 ; Salisbury, P. 3 ; Banga, S. S. 4 ; Banga, Shashi 4 ;

作者机构： 1.Univ Western Australia, Fac Nat & Agr Sci, Sch Plant Biol, Perth, WA 6009, Australia

2.Univ Western Australia, Fac Nat & Agr Sci, Sch Earth & Geog Sci, Perth, WA 6009, Australia

3.Univ Melbourne, Sch Agr & Food Syst, Melbourne, Vic 3010, Australia

4.Punjab Agr Univ, Genet & Biotechnol, Dept Plant Breeding, Ludhiana 141004, Punjab, India

5.Natl Res Ctr Rapeseed Mustard, Bharatpur 321303, India

6.Haryana Agr Univ, CCS, Oilseeds Sect, Hisar 125004, Haryana, India

7.Energy & Resources Inst, New Delhi 110003, India

8.Chinese Acad Agr Sci, Inst Oil Crop

期刊名称：AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH （ 影响因子：1.328； 五年影响因子：1.781 ）

ISSN： 0004-9409

年卷期： 2007 年 58 卷 12 期

页码：

收录情况： SCI

摘要： Sclerotinia stem rot (SSR) is a significant agricultural problem worldwide. Finding sources of resistance is crucial to the ongoing search for better management of this disease. Brassica germplasm from Australia, China and India was screened for resistance to SSR under Western Australian field conditions following stem inoculation, application of a spray of mycelial suspension, or as a consequence of myceliogenic germination originating from sclerotia resident in soil. Significant differences in response were observed among 53 genotypes using each of the three screening methods. There was a variable impact of the time of inoculation on the disease level depending upon time of assessment post-stem inoculation. However, this impact could be reduced to an insignificant level provided the assessment after stem inoculation was delayed until 3 weeks post-inoculation. The results of these studies indicate that the use of appropriate inoculation and assessment methods could significantly reduce variability in the responses commonly observed in screening for resistance in crop plants against Sclerotinia sclerotiorum.

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