Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid between N-tabacum and N-glauca
文献类型： 外文期刊
作者： Sun, Y. 1 ; Xue, Q. 2 ; Zhang, X. 2 ; Ding, C. 2 ; Zhang, L. 2 ; Gong, M. 2 ; Bu, G. 3 ; Wang, W.;
作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310029, Peoples R China
2.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310029, Peoples R China; Chinese Acad Agr Sci, Qingzhou Tabacco Res Inst, Shandong, Peoples R China
3.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310029, Peoples R China; Chinese Acad Agr Sci, Qingzhou Tabacco Res Inst, Shandon
关键词： N. tabacum;N. glauca;somatic hybrid;RAPD;SCAR
期刊名称：PLANT BIOSYSTEMS （ 影响因子：2.838； 五年影响因子：2.146 ）
ISSN： 1126-3504
年卷期： 2007 年 141 卷 2 期
页码：
收录情况： SCI
摘要： By using the protoplast fusion technique, we have obtained 44 regenerated plants, phenotypically different and distinct from their parents, among which we have identified a fertile symmetric somatic hybrid, designated as TG-32, between N. tabacum var. Gexin No. 1 and N. glauca. The morphology, fertility, chromosome number and nuclear constitution of the somatic hybrid have been studied in detail. Unlike other asymmetric interspecific somatic hybrids, the chromosome number of the symmetric somatic hybrid is 72, equal to the sum of chromosomes of both parents. The TG-32 plant has flowers similar to those of N. tabacum, but with petals similar to those of N. glauca. Interestingly the offspring of TG-32 vary in seed production ability with temperature, and produce more seeds under a relatively low temperature. Two SCAR markers were used to evaluate genetic variability and structure. The hybrid amplified the expected fragment, but the parents showed only one of two markers. This experimental result supports the hypothesis of the co-existence of two parental genomes in the somatic hybrid.
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