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Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization

文献类型： 外文期刊

作者： Liu, M. Y. 1 ; Wang, X. L. 2 ; Fu, B. Q. 2 ; Li, C. Y. 3 ; Wu, X. P.; Le Rhun, D.; Chen, Q. J.; Boireau, P.;

作者机构： 1.Jilin Univ, Minist Educ, Inst Zoonoses, Key Lab Zoonoses, Changchun 130062, Peoples R China

2.Jilin Univ, Minist Educ, Inst Zoonoses, Key Lab Zoonoses, Changchun 130062, Peoples R China; Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Province, Lanzhou 730046, Peoples R China; Swedish Inst Infect Dis Control, Stockholm, Sweden; UPUM, ENYA, AFSSA, INRA,UMR 956, F-94703 Maisons Alfort, France

3.Jilin Univ, Minist Educ, Inst Zoonoses, Key Lab Zoonoses, Changchun 130062, Peoples R China; Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Province, Lanzhou 730046,

关键词： Trichinella spiralis;stage-specifically expressed genes;newborn larvae;adults;suppression subtractive hybridization

期刊名称：PARASITOLOGY （ 影响因子：3.234； 五年影响因子：3.118 ）

ISSN： 0031-1820

年卷期： 2007 年 134 卷

页码：

收录情况： SCI

摘要： Newborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families of Trichinella spiralis were identified by suppression subtractive hybridization (SSH)dagger. Six cDNA clones were identified as NBL stage-specific, including 1 member of the T. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease 11 (DNase I I)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified.

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