文献类型： 外文期刊
作者： Heng, W. 1 ; Wu, H. Q. 1 ; Huang, S. X. 1 ; Zhang, S. J. 1 ; Wu, J. 1 ; Fang, C. Q. 2 ; Zhang, S. L. 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Agr Sci, Fruit Res Inst, Xingcheng 125100, Liaoning, Peoples R China
期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）
ISSN： 1462-0316
年卷期： 2008 年 83 卷 5 期
页码：
收录情况： SCI
摘要： Chinese pear (Pyrus spp.) exhibits gametophytic self-incompatibility (GSI), as do other fruit species in the family Rosaceae. This work determined S-locus diversity in 20 native Chinese pear cultivars or wild accessions using polymerase chain reaction (PCR) and pollination experiments. After cloning and sequencing the PCR products, the S-genotypes of all 20 pear cultivars or wild accessions were determined. Subsequent sequence analysis showed that P. sinkiangensis Yu 'Aolian' (S(p)S(32)), P. phaeocarpa Rehd. 'Diaodan' (S(d)S(e)), P. xerophila Yu 'Shageda' (S(36)S(d)) and 'Xingyeli' (S(22)S(c)), originating in China, shared some S-RNase genes with P communis, providing evidence that oriental and occidental Pyrus species may share the same pool of alleles at the S-locus. Two novel S-RNase genes were also discovered in P. ussuriensis 'Maili' and 'Neimenggushanli', and deposited as S(40) and S(41), under the accession numbers DQ903313 and DQ988687, respectively. Their deduced amino acid sequences showed high similarity to S(11)-RNase (100%) and S(6)-RNase (94.4%) between the C1 and C3 exons in Malus. The high similarity scores between S-RNases in Pyrus and Malus indicate that the existence of S-RNases predated speciation between Pyrus and Malus.
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