Effects of copper exposure and recovery in juvenile yellowtail kingfish (Seriola lalandi): Histological, physiological and molecular responses
文献类型: 外文期刊
作者: Jin, Zhixin 1 ; Xu, Yongjiang 1 ; Zhou, Heting 1 ; Cui, Aijun 1 ; Jiang, Yan 1 ; Wang, Bin 1 ; Zhang, Wenjing 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Joint Lab Deep Blue Fishery Engn, Qingdao, Peoples R China
3.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China
关键词: Seriola lalandi; Copper ecotoxicity; Histopathology; Enzymatic activity; Transcriptome analysis
期刊名称:AQUACULTURE REPORTS ( 影响因子:3.7; 五年影响因子:3.8 )
ISSN: 2352-5134
年卷期: 2023 年 31 卷
页码:
收录情况: SCI
摘要: This study investigated the physiological responses to copper toxicity in liver and gill of juvenile yellowtail kingfish by exposing to 0 (control), 0.0672, 0.12, 0.214, 0.384, 0.672, 1.20, or 2.14 mg/L copper sulfate for 7 days and then recovered for 14 days. The histopathology, enzyme activities and differential gene expression were detected. The results indicate the safe concentration value of copper for juvenile yellowtail kingfish is 0.083 mg/ L. Copper exposure caused structural damage to gill and liver, and could be disappeared or restored after the recovery period. Copper exposure enhanced the Cu,Zn superoxide dismutase (Cu/Zn-SOD) and glutathione peroxidase (GSH-Px) activities and the malondialdehyde (MDA) level in liver. However, with increasing copper concentrations, liver aspartate transaminase (AST) and gill Na+/K+-ATPase activities were at first enhanced and then inhibited. Serum lysozyme (LZM) and Cu/Zn-SOD activities were enhanced at low copper concentrations but inhibited at high concentrations, and the activities of alanine transaminase (ALT) and AST significantly increased at greater copper concentrations. After the recovery period, only liver Cu/Zn-SOD activity returned to control level. In contrast, liver GSH-Px activity and MDA levels and gill Na+/K+-ATPase activity, serum LZM and AST activities remained significantly higher than control, but liver AST activity, serum ALT, and Cu/Zn-SOD activities were significantly lower than control. We constructed the gene regulatory networks of molecular pathways in liver and gill in response to copper exposure. The DEGs were enriched mainly in the pathway of lipid metabolism in liver and pathway of oxidative phosphorylation in gill, indicating these respective pathways are involved in the response to copper stress in this species. The present results elucidate some histological, physiological, and molecular mechanisms underlying copper-exposure stresses in yellowtail kingfish, which could help guide the safe utilization of copper sulfate in disease control of farmed individuals.
- 相关文献
作者其他论文 更多>>
-
Molecular characterization and ontogenetic expression profiles of LPXRFa and its receptor in Japanese flounder (Paralichthys olivaceus)
作者:Chen, Jun;Li, Yuru;Zhang, Wenwen;Zhao, Limiao;Huang, Xueying;Fang, Yan;Wang, Bin;Wang, Bin;Chen, Jun;Wang, Bin
关键词:LPXRFa; LPXRFa-R; Ontogenesis; Japanese flounder
-
Responses of the gastrointestinal microbiota to the protein metabolism of pond-cultured Japanese flounder (Paralichthys olivaceus)
作者:Jiang, Yan;Xu, Yongjiang;Cui, Aijun;Wang, Kaijie;Liu, Xuezhou;Wang, Bin;Wang, Jun;Lin, Lin
关键词:Paralichthys olivaceus; protein metabolism; protease activity; gene expression; gastrointestinal microbiota
-
Histological, microecological and transcriptomic physiological responses underlying hypoxia and reoxygenation adaptation in yellowtail kingfish (Seriola lalandi)
作者:Zhou, Heting;Jiang, Yan;Xu, Yongjiang;Cui, Aijun;Feng, Yuan;Jin, Zhixin;Wang, Bin;Zhou, Heting;Feng, Yuan
关键词:Seriola lalandi; hypoxia and reoxygenation; gastrointestinal microbiota; lipid metabolism; gill remodeling
-
Differential activation of neuropeptide FF receptors by gonadotropin-inhibitory hormone peptides in the European sea bass
作者:Wang, Bin;Wang, Bin;Wang, Bin;Paullada-Salmeron, Jose A.;Verges-Castillo, Alba;Munoz-Cueto, Jose A.;Paullada-Salmeron, Jose A.;Verges-Castillo, Alba;Munoz-Cueto, Jose A.;Paullada-Salmeron, Jose A.;Verges-Castillo, Alba;Munoz-Cueto, Jose A.;Paullada-Salmeron, Jose A.;Verges-Castillo, Alba;Munoz-Cueto, Jose A.
关键词:GnIH; NPFF; NPAF; NPFF receptor; signaling pathway
-
Comparative Analysis of Genetic Structure and Diversity in Five Populations of Yellowtail Kingfish (Seriola aureovittata)
作者:Cui, Aijun;Xu, Yongjiang;Jiang, Yan;Wang, Bin;Liu, Xuezhou;Cui, Aijun;Xu, Yongjiang;Jiang, Yan;Wang, Bin;Liu, Xuezhou;Kikuchi, Kiyoshi;Koyama, Takashi
关键词:Seriola aureovittata; population genetics; single nucleotide polymorphisms; 2b-RAD sequencing
-
Food deprivation differentially modulates gene expression of LPXRFa and kisspeptin systems in the brain-pituitary axis of half-smooth tongue sole (Cynoglossus semilaevis)
作者:Wang, Bin;Cui, Aijun;Xu, Yongjiang;Zhang, Yaxing;Jiang, Yan;Liu, Xuezhou;Wang, Bin;Cui, Aijun;Xu, Yongjiang;Jiang, Yan;Liu, Xuezhou
关键词:LPXRFa; LPXRFa receptor; kisspeptin; kisspeptin receptor; Cynoglossus semilaevis
-
New Evidence for the Existence of Two Kiss/Kissr Systems in a Flatfish Species, the Turbot (Scophthalmus maximus), and Stimulatory Effects on Gonadotropin Gene Expression
作者:Zhao, Chunyan;Wang, Wenqi;Zhao, Chunyan;Liu, Yifan;Feng, Chengcheng;Xu, Shihong;Liu, Qinghua;Li, Jun;Zhao, Chunyan;Liu, Yifan;Feng, Chengcheng;Xu, Shihong;Liu, Qinghua;Li, Jun;Wang, Bin;Liu, Yifan;Li, Jun
关键词:turbot; kisspeptin; kisspeptin receptor; reproduction; gonadal development