文献类型: 外文期刊
作者: Zhang, C. X. 1 ; Zhao, X. 1 ; Han, F. 1 ; Yang, M. F. 1 ; Chen, H. 1 ; Chida, T. 3 ; Shen, S. H. 1 ;
作者机构: 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Agr Sci, Res Inst Pomol, Beijing 125100, Peoples R China
3.Chem Agrochem Planning & Dev Dept, Tokyo 1038552, Japan
关键词: Bacillus subtilis;Magnaporthe grisea P131;in vitro antifungal activity;proteomics;2DE-PAGE
期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )
ISSN: 1017-7825
年卷期: 2009 年 19 卷 4 期
页码:
收录情况: SCI
摘要: Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.
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