文献类型： 外文期刊

作者： Pu Xiu-ying ² ; Liang Jian-ping ¹ ; Shang Ruo-feng ³ ; Wang Xue-hong ³ ; Wang Zuo-xin ² ; Hua Lan-ying ³ ; Liu Yu ³ ;

作者机构： 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China

2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

3.Chinese Acad Agr Sci, Lanzhou Inst Anim & Vet Pharmaceut Sci, Lanzhou 730050, Peoples R China

关键词： porcine respiratory and reproductive syndrome virus (PRRSV);porcine respiratory and reproductive syndrome (PRRS);Hypericum perforatum extract (HPE);piglet

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 6 期

页码：

收录情况： SCI

摘要： To study the influence of Hypericum perforatum extract (HPE) on piglets infected with porcine respiratory and reproductive syndrome virus (PRRSV), enzyme-labeled immunosorbent assay (ELISA) and cytopathic effect (CPE) were used to determine in vitro whether HPE could induce swine pulmonary alveolar macrophages (PAMs) to secrete IFN-gamma, and whether PRRSV titers in PAMs were affected by the levels of HPE-induced IFN-gamma. HPE (200 mg kg(-1)) was administrated by oral gavage to piglets infected with the PRRSV in vivo to observe whether HPE affected the viremia, lung viral titers, and weight gain of piglets infected with PRRSV. The results showed that HPE was capable of inducing PAMs to produce IFN-gamma in a dose dependent manner and HPE pretreatment was capable of significantly reducing PRRSV viral titers in PAMs (P<0.01). Administration of HPE to the PRRSV-infected animals significantly (P<0.05) reduced viremia over time as compared with the PRRSV-infected animals. But there was not significant decrease in lung viral titers at day 21 post-infection between the HPE-treated animals and the PRRSV-infected control piglets. There were no significant differences in weight gain over time among the HPE-treatment animals, the normal control, and the HPE control animals. The PRRSV-infected animals caused significant (P<0.01) growth retardation as compared with the HPE controls and the normal piglets. It suggested that HPE might be an effective novel therapeutic approach to diminish the PRRSV-induced disease in swine.