您好,欢迎访问中国农业科学院 机构知识库!

Development of Sequence Characterized Amplified Region (SCAR) Primers for the Detection of Resistance to Sporisorium reiliana in Maize

文献类型: 外文期刊

作者: Shi Hong-liang 1 ; Li Xin-hai 2 ; Zhang De-gui 1 ; Xie Chuan-xiao 2 ; Hao Zhuan-fang 2 ; Li Ming-shun 2 ; Pan Guang-ta 1 ;

作者机构: 1.Sichuang Agr Univ, Maize Res Inst, Yaan 625014, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

关键词: maize (Zea mays L.);Sporisorium reiliana;bulked segregant analysis;amplified fragment length polymorphism;sequence characterized amplified region

期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )

ISSN: 1671-2927

年卷期: 2009 年 8 卷 8 期

页码:

收录情况: SCI

摘要: Head smut of maize (Zea mays L.), which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of resistance genes and MAS. Two sets of BC3 progenies, one (BC3Q) derived from the cross Qi319 (resistance) x Huangzao 4 (susceptible), the other (BC3M) from Mo17 (resistance) x Huangzao 4 (susceptible), were generated. Huangzao 4 was the recurrent parent in both progenies. A combination of BSA (bulked segregant analysis) with AFLP (amplified fragment length polymorphism) method was applied to map the genes involving the resistance to S. reiliana, and corresponding resistant and susceptible bulks and their parental lines were used for screening polymorphic AFLP primer pairs. One fragment of PI3M61-152 was converted into SCAR (sequence charactered amplified fragment) marker S130. The marker was mapped at chromosome bin 2.09, the interval of a major QTL region previously reported to contribute to S. reiliana resistance. Furthermore. S130 was highly associated with resistance to S. reiliana, and could be useful for marker-assisted selection and facilitate map-based cloning of resistance genes.

  • 相关文献

[1]Analysis of QTL for resistance to head smut (Sporisorium rediana). Li, X. H.,Gao, S. R.,Shi, H. L.,Zhang, S. H.,Li, M. S.,Xie, C. X.,Wang, Z. H.,George, M. L. C.. 2008

[2]Genetic diversity in a collection of Chinese maize inbred lines for resistance to head smut caused by Sporisorium reiliana. Wang, Z. H.,Li, X. H.,Xie, C. X.,Li, M. S.,Hao, Z. F.,Xiao, M. J.,Gao, S. R.,Zhang, S. H.,Wang, Z. H.,George, M. L. C.. 2008

[3]Two RAPD markers linked to a major fertility restorer gene in pepper. Zhang, BX,Huang, SW,Yang, GM,Guo, JZ. 2000

[4]Effect of the puroindoline locus and environment on Chinese fresh noodle texture. Yang, EN,Zou, YC,Chen, DS,Sheppard, J,Martin, D,Huang, S,Mares, D,Sutherland, MW. 2006

[5]Bulked sample analysis in genetics, genomics and crop improvement. Zou, Cheng,Wang, Pingxi,Xu, Yunbi,Xu, Yunbi.

[6]QTL mapping of adult-plant resistance to stripe rust in a "Lumai 21xJingshuang 16' wheat population. He, Zhong H.,Xia, Xian C.,Ren, Yan,Liu, Li S.,He, Zhong H.,Wu, Ling,Bai, Bin.

[7]Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array. Lu, Yuqing,Wu, Xiaoyang,Yao, Miaomiao,Zhang, Jinpeng,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Du, Juan,Gao, Ainong,Li, Lihui.

[8]Isolation and identification of a gene in response to rice blast disease in rice. Zheng, XW,Chen, XW,Zhang, XH,Lin, ZZ,Shang, JJ,Xu, JC,Zhai, WX,Zhu, LH.

[9]Characterization and fine mapping of a novel barley Stage Green-Revertible Albino Gene (HvSGRA) by Bulked Segregant Analysis based on SSR assay and Specific Length Amplified Fragment Sequencing. Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Guo, Ganggang,Xu, Yuxin. 2015

[10]Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus). Zhang, H.,Sun, R.,Zhang, H.,Strelkov, S. E.,Feng, J.,Hwang, S. -F.,Falak, I.,Huang, X..

[11]Genetic Diversity of Rice False Smut Fungus, Ustilaginoidea virens and its Pronounced Differentiation of Populations in North China. Zhou, Y. -L.,Pan, Y. -J.,Xie, X. -W.,Zhu, L. -H.,Xu, J. -L.,Li, Z. -K.,Li, Z. -K.,Wang, S..

[12]Chromosome elimination, addition and introgression in intertribal partial hybrids between Brassica rapa and Isatis indigotica. Tu, Yuqin,Sun, Jian,Ge, Xianhong,Li, Zaiyun,Sun, Jian.

[13]Genetic and histological characterization of a novel recessive genic male sterile line of Brassica napus derived from a cross with Capsella bursa-pastoris. Chen, Hai-Feng,Ge, Xian-Hong,Du, Xue-Zhu,Zhao, Zhi-Gang,Li, Zai-Yun,Chen, Hai-Feng.

[14]Evidence of genetic recombination in wheat yellow rust populations of a Chinese oversummering area. Mboup, M.,Leconte, M.,Gautier, A.,de Vallavieille-Pope, C.,Enjalbert, J.,Wan, A. M.,Chen, W.,Wan, A. M..

[15]Development of a sequence-characterized amplified region marker for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kuhn. Gao, L.,Liu, T. G.,Chen, W. Q.. 2009

[16]Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers. Hao, Zhuanfang,Li, Xinhai,Xie, Chuanxiao,Weng, Jianfeng,Li, Mingshun,Zhang, Degui,Liu, Lingling,Liu, Sisi,Zhang, Shihuang,Liang, Xiaoling. 2011

[17]Stability of QTL Across Environments and QTL-by-Environment Interactions for Plant and Ear Height in Maize. Zhang Yan,Li Yong-xiang,Wang Yang,Liu Zhi-zhai,Peng Bo,Tan Wei-wei,Wang Di,Shi Yun-su,Song Yan-chun,Wang Tian-yu,Li Yu,Liu Cheng,Sun Bao-cheng,Liu Zhi-zhai. 2010

[18]Effects of Shading at Different Stages After Anthesis on Maize Grain Weight and Quality at Cytology Level. Jia Shi-fang,Li Cong-feng,Dong Shu-ting,Zhang Ji-wang,Jia Shi-fang,Li Cong-feng. 2011

[19]Bulked Segregant RNA-seq Reveals Differential Expression and SNPs of Candidate Genes Associated with Waterlogging Tolerance in Maize. Du, Hewei,Wang, Hongwei,Ding, Shuangcheng,Zhang, Binglin,Tian, Xiaohai,Xu, Yunbi,Du, Hewei,Zhu, Jianxiong,Su, Hang,Huang, Ming,Luo, An,Wei, Shudong,Du, Hewei,Xu, Yunbi,Xu, Yunbi. 2017

[20]Trends of grain yield and plant traits in Chinese maize cultivars from the 1950s to the 2000s. Ci, Xiaoke,Li, Mingshun,Xu, Jiashun,Lu, Zhenyu,Bai, Pengfei,Ru, Gaolin,Zhang, Degui,Li, Xinhai,Bai, Li,Xie, Chuanxiao,Hao, Zhuanfang,Zhang, Shihuang,Ci, Xiaoke,Dong, Shuting,Liang, Xiaoling.

作者其他论文 更多>>