您好,欢迎访问中国农业科学院 机构知识库!

Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis

文献类型: 外文期刊

作者: Zhou GuoAn 1 ; Guan RongXia 1 ; Li YingHui 1 ; Chang RuZhen 1 ; Qiu LiJuan 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Germplasm & Biotechnol MOA, Beijing 100081, Peoples R China

关键词: GmNHX2;Na+/H+ antiporter;salt tolerance;soybean;ion homeostasis

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2009 年 54 卷 19 期

页码:

收录情况: SCI

摘要: Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

  • 相关文献

[1]Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance. Wang, Maoyan,Gu, Dan,Liu, Tingsong,Wang, Zhaoqiang,Guo, Xiying,Hou, Wei,Bai, Yunfeng,Chen, Xiaoping,Wang, Guoying.

[2]GmSALT3, Which Confers Improved Soybean Salt Tolerance in the Field, Increases Leaf Cl- Exclusion Prior to Na+ Exclusion But Does Not Improve Early Vigor under Salinity. Liu, Ying,Yu, Lili,Chen, Jingling,Liu, Xiexiang,Hong, Huilong,Liu, Zhangxiong,Chang, Ruzhen,Qiu, Lijuan,Guan, Rongxia,Qu, Yue,Gilliham, Matthew,Qu, Yue,Gilliham, Matthew. 2016

[3]Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment. Zhao, Shu-Ping,Zheng, Wei-Jun,Zhao, Wan,Yu, Tai-Fei,Zhou, Yong-Bin,Min, Dong-Hong,Chai, Shou-Cheng,Zhang, Xiao-Hong,Zhao, Shu-Ping,Xu, Zhao-Shi,Zhao, Wan,Chen, Ming,Ma, You-Zhi,Wang, Yan-Xia. 2017

[4]Identification and Functional Analysis on Abiotic Stress Response of Soybean Cl- Channel Gene GmCLCnt. Zhou Guo-an,Qiu Li-juan. 2010

[5]Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants. Cao, Dong,Liu, Xiaobing,Cao, Dong,Hou, Wensheng,Liu, Wei,Yao, Weiwei,Wu, Cunxiang,Han, Tianfu,Cao, Dong. 2011

[6]异源表达一个大豆Na~+/H~+逆向转运蛋白基因GmNHX2提高拟南芥的耐盐性. 周国安,关荣霞,李英慧,常汝镇,邱丽娟. 2009

[7]Expression of tomato SlTIP2;2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins. Xin, Shichao,Yu, Guohong,Qiang, Xiaojing,Xu, Na,Cheng, Xianguo,Sun, Linlin.

[8]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. QINGCHUAN YANG,MINGSHENG WU,PINGQING WANG,JUNMEI KANG,XINGLONG ZHOU. 2010

[9]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. Yang, QC,Wu, MS,Wang, PQ,Kang, JM,Zhou, XL.

[10]Effect of salt stress on the growth, Na+, Cl−, glycinebetaine and proline accumulation in sugar beet seedlings. Gui Geng,Lihua Yu,Cuibing Zheng,Zhi Pi,Chunxue Peng,Yun Yang. 2011

[11]Perspective on the Trend of Soybean Production and Trade in China. Qing Zhang,Lifeng Liu. 2014

[12]Exchanging, preserving and characterizing germplasm in China. L. Qiu,R. Chang. 2004

[13]INHERITANCE OF A NDLL-ALLELE FOR LIPOXYGENASE- 2.3 AND KUNITZ TRYPSIN INHIBITOR IN SOYBEAN. Sun Junming,Ding Anlin,Han Fenxia. 2001

[14]Co-expression of AtNHX1 and TsVP improves the salt tolerance of transgenic cotton and increases seed cotton yield in a saline field. Cheng Cheng,Zhang, Kewei,Ying Zhang,Xiugui Chen,Jiuling Song,Zhiqiang Guo,Kunpeng Li,Kewei Zhang. 2018

[15]Genotypic variations in ion homeostasis, photochemical efficiency and antioxidant capacity adjustment to salinity in cotton (Gossypium hirsutum L.). Ning Wang,Haikun Qi,Guilan Su,Jie Yang,Hong Zhou,Qinghua Xu,Qun Huang,Gentu Yan.

[16]iTRAQ-Based Comparative Proteomic Analysis of Seedling Leaves of Two Upland Cotton Genotypes Differing in Salt Tolerance. Wenfang Gong,Feifei Xu,Du, Xiongming,Junling Sun,Zhen Peng,Shoupu He,Zhaoe Pan,Xiongming Du. 2017

[17]Characterization of Salt-Induced Epigenetic Segregation by Genome-Wide Loss of Heterozygosity and its Association with Salt Tolerance in Rice (Oryza sativa L.). Li, Min,Li, Min,Wang, Wen-Sheng,Pang, Yun-Long,Xu, Jian-Long,Fu, Bin-Ying,Li, Zhi-Kang,Li, Min,Pang, Yun-Long,Domingo, Jessica R.,Ali, Jauhar,Venus, Elec B.,Li, Min,Xu, Jian-Long,Fu, Bin-Ying,Li, Zhi-Kang. 2017

[18]The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco. Zhang, HW,Huang, ZJ,Xie, BY,Chen, Q,Tian, X,Zhang, XL,Zhang, HB,Lu, XY,Huang, DF,Huang, RF. 2004

[19]Seedling salt tolerance in tomato. Li, Junming,Liu, Lei,Zhang, Pujuan,Du, Yongchen,Li, Junming,Bai, Yuling,Finkers, Richard,Visser, Richard G. F.,van Heusden, Adriaan W.,Li, Junming. 2011

[20]Comparison of Salt Tolerance in Soja Based on Metabolomics of Seedling Roots. Li, Mingxia,Jiao, Yang,Zhang, Haiyan,Shi, Lianxuan,Guo, Rui,Jin, Xiaofei. 2017

作者其他论文 更多>>