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Correlation Analysis Between Single Nucleotide Polymorphisms in Exon 2 of ADSL Gene and Inosine Monophosphate Acid Content in Chicken

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文献类型：外文期刊

作者： Wu, X. S. ² ; Bao, W. B. ² ; Shu, J. T. ² ; Xu, Q. ² ; Zhang, X. Y. ² ; Han, W. ² ; Chen, G. H. ¹ ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Inst Poultry Sci, Yangzhou 225003, Peoples R China

关键词： ADSL gene;SNP;inosine monophosphate content;chicken;genotype;Hardy-Weinberg equilibrium

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （影响因子：0.365；五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2009 年 8 卷 12 期

页码：

收录情况： SCI

摘要： This study was designed to investigate the effect of Adenylosuccinate Lyase (ADSL) gene on Inosine Monophosphate content (IMP) in chicken. The chickens used for the study included exogenous Recessive White chicken and Chinese indigenous chicken breeds such as Silkies, Baier, Tibetan and Xiaoshan chickens. A pair of primer was designed to detect exon 2 of ADSL gene by PCR-SSCP and mutation detected was directly sequenced. A synonymous mutation at 3484 bp in exon 2 was substituted C/T SNP. In five chicken breeds, three genotypes TT, CT and CC were observed in exon 2. Muscle IMP content of individuals with T-F genotype in exon 2 had significantly higher than those with CT and CC genotypes (p<0.05). In exon 2, Recessive White, Xiaoshan and Baier chickens were in Hardy-Weinberg equilibrium (p>0.05) and Silkies and Tibetan chickens were significant departure from Hardy-Weinberg equilibrium (p<0.01).

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