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Gene Cloning and Tissue-Specific Expression of G Protein beta Subunit in Microplitis mediator (Hymenoptera: Braconidae)

文献类型： 外文期刊

作者： Zhang Shuai 1 ; Zhang Yong-jun 1 ; Cui Jin-jie 1 ; Gao Xi-wu 1 ; Guo Yu-yuan 1 ;

作者机构： 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

3.Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

4.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China

关键词： Microplitis mediator;G protein beta subunit;quantitative real-time RT-PCR;expression pattern

期刊名称：Agricultural Sciences in China （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 4 期

页码：

收录情况： SCI

摘要： A gene encoding a novel G protein beta subunit of beta 1 subclass, G beta Mmed was isolated from Microfilms mediator (Hymenoptera. Braconidae) The full-length sequence of G beta Mmed is 1 119 bp, the cDNA contains a 1 023 bp open reading frame that encodes a protein with 340 ammo acids, and the predicted molecular weight of G beta Mmed is 37 23 kDa and isoelectric point is 5 86. By the quantitative real-time RT-PCR method, the tissue-specific expression and quantitative changes in the developmental expression profile of G beta Mmed were detected It was found that G beta Mmed was abundantly expressed in M. mediator antennae, head (without antennae), thorax, abdomen, legs and the wings. and especially at high levels in abdomen. In antennae, expression varied through 1st day before emergence to 5-d-old adults, and had equal expression levels detected in females and males in total In head, G beta Mmed expresses while initially high in females, and have another peaked in stage 4 and 1st day. in males showed a peak of G beta Mmed expression prior to emergence and relatively low levels after emergence In female abdomen G beta Mmed expression levels have two peaks in stage 1 and the 5th d, but just have one peak in male abdomen in stage 1 In all other tissues expression was low and stable

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