文献类型： 外文期刊

作者： Pu Zhong-hui ¹ ; Zhang Yu-qun ¹ ; Yin Zhong-qiong ¹ ; Xu Jiao ¹ ; Jia Ren-yong ¹ ; Lu Yang ¹ ; Yang Fan ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Anim Med, Yaan 625014, Peoples R China

2.Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Yaan 625014, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China

4.China Agr Univ, Key Lab Herb Med Vet Chem Med & Safety Evaluat, Minist Agr, Coll Vet Med, Beijing 100193, Peoples R China

关键词： Azadirachta indica A. Juss;neem oil;MIC;MBC;time-inhibition curve;9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 8 期

页码：

收录情况： SCI

摘要： The 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester from neem oil was investigated for antibacterial activity against three bacterial strains viz., Staphylococcus aureus ATCC No. 25923, Escherichia coli ATCC No. 44102 and Salmonella sp. ATCC No. 50 041 in vitro. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester were determined by using the broth microdilution dilution (BMD) method at different concentrations ranging from 20 to 0.625 mg mL(-1). Its time-inhibition curve against E. coli was also tested and showed that the MIC values for the bacterial strains S. aureus, E. coli and Salmonella sp. were 20, 5 and 10 mg mL(-1), respectively. Its MBC values were 20, 20 and 10 mg mL(-1), respectively. The antibacterial activity of 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester against three strain tested showed the relationship with time and concentration.