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Cloning and Characterization of a Putative CTR1 Gene from Wheat

文献类型： 外文期刊

作者： Bi Cai-Li 1 ; Wen Xiao-Jie 1 ; Zhang Xue-Yong 1 ; Liu Xu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Germplasm Resources & Biotechnol, Beijing 100081, Peoples R China

2.Hebei Normal Univ, Life Sci Acad, Shijiazhuang 050016, Peoples R China

关键词： CTR1;RACE;stress;Triticum aestivum L.;transgenic tobacco

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 9 期

页码：

收录情况： SCI

摘要： CTR1 is a key negative regulator in ethylene signal transduction. A salt-induced CTR1 like gene (TaCTR1) was cloned from wheat, its expression under abiotic stresses, subcellular localization and the effect of overexpression of TaCTR1 on salt tolerance in tobacco was studied. A putative CTRI gene was cloned and characterized from wheat via rapid amplification of cDNA ends (RACE) and RT-PCR. TaCTR1 expression under stresses was analyzed using semi-quantitative RT-PCR and the effect of overexpression of TaCTR1 on salt tolerance was conducted in tobacco. The full-length cDNA of TaCTR1 is 2 635 bp which codes for a polypeptide of 759 amino acids. There is a conserved serine/threonine protein kinase domain at the carboxyl terminus containing an ATP-binding site. Southern blot analysis revealed that TaCTR1 consisted of a gene family in wheat. The amino acid homologies of CTR1 among different organisms share higher similarities. Expression analysis revealed that TaCTR1 was induced by NaCl and drought stress but inhibited by ABA treatment. Transient expression of TaCTR1-GFP in the onion epidermal cells indicated that TaCTR1 was probably targeted to the plasma membrane. Overexpression of TaCTR1 decreased salt tolerance in transgenic tobacco (Nicotiana tabacum L.) plants compared with the control. To our knowledge, TaCTR1 is the first CTR1 gene cloned in wheat and may be involved in various abiotic stresses. Overexpression of TaCTR1 decreased the salt tolerance in tobacco suggested that TaCTR1 may act as a negative regulator of salt stress in plants.

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