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Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds

文献类型： 外文期刊

作者： Sun Jun-ming 1 ; Sun Bao-li 2 ; Han Fen-xia 1 ; Yan Shu-rong 1 ; Yang Hua 1 ; Kikuchi, Akio 3 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Inst Agr Environm & Sustainable Dev, Beijing 100081, Peoples R China

3.Natl Agr Res Ctr Tohoku Reg, Akita 0192112, Japan

关键词： soybean (Glycine max L. Merri);isoflavone;HPLC

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 1 期

页码：

收录情况： SCI

摘要： It is important to determine the isoflavone components by high-performance liquid chromatography (HPLC) for the molecular assistant selection of isoflavone in soybean. Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. The results showed that there were different maximum ultraviolet (UV) absorbance for the aglycones of daidzein, glycitein, and genistein, which were at 250, 257, and 260 nm, respectively. A linear gradient elution of acetonitrile (13-30%) containing 0. 1% acetic acid as a mobile phase was applied on a YMC-C18 column at 35 degrees C. The 12 isoflavone components were determined using the UV detector by HPLC. We concluded that this is a rapid and precise method which adapted to determine the large numbers of samples with microanalysis.

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