您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genetic variation of the 8-kDa glycoprotein family from Echinococcus granulosus, Taenia multiceps and Taenia hydatigena

文献类型： 外文期刊

作者： Jia Wan-zhong 1 ; Yan Hong-bin 1 ; Lou Zhong-zi 1 ; Ni Xing-wei 1 ; Liu Hong-xia 1 ; Li Hong-min 2 ; Guo Ai-jiang; Fu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol,Minist Agr, Key Lab Vet Publ Hlth,Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

2.Chinese Acad Agr Sci, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol,Minist Agr, Key Lab Vet Publ Hlth,

关键词： Echinococcus granulosus;Taenia multiceps;Taenia hydatigena;8-kDa glycoprotein;genetic variation

期刊名称：CHINESE MEDICAL JOURNAL （ 影响因子：2.628； 五年影响因子：2.361 ）

ISSN： 0366-6999

年卷期： 2011 年 124 卷 18 期

页码：

收录情况： SCI

摘要： Background Echinococcosis, coenurosis and cysticercosis are debilitating diseases which prevail in China. Immunological diagnosis of metacestodosis is important in disease control. The 8-kDa glycoproteins from taeniid cestodes have successfully been used for diagnosis of human cysticercosis in immunological assays. The aim of the present study was to investigate genetic variations and phylogenetic relationships of the 8-kDa proteins for evaluating the possibility of utilizing these proteins as diagnostic antigens for other metacestode infections. Methods The genes and complementary DNAs (cDNAs) encoding the 8-kDa proteins from Echinococcus (E.) granulosus, Taenia (T.) multiceps and T. hydatigena were amplified using PCR method. Their amplicons were cloned into the vector pMD18 and the positive clones were sequenced. Sequence data were analyzed with the SeqMan program, and sequence homology searches were performed using the BLAST program. Alignments were conducted using the ClustalX program, and the phylogenetic analyses were performed with the Protein Sequences Program and the Puzzle Program using the Neighbor-joining method. Results Fifteen, 18 and 22 different genomic DNA sequences were identified as members of the 8-kDa protein gene family from E. granulosus, T. multiceps and T. hydatigena, respectively. Eight, four and six different cDNA clones respectively from E. granulosus, T. multiceps and T. hydatigena were characterized. Analysis of these sequences revealed 54 unique 8-kDa protein sequences. Phylogenetic trees demonstrated that the taeniid 8-kDa proteins are clustered into eight clades at least: Ts18, Ts14, TsRS1, TsRS2, T8kDa-1, T8kDa-2, T8kDa-3 and T8kDa-4. Conclusion We found that the gene family encoding for the taeniid 8-kDa antigens is comprised of many members with high diversity, which will provide molecular evidence for cross-reaction or specific reaction among metacestode infections and may contribute to the development of promising immunological methods for diagnosis of metacestodosis. Chin Med J 2011;124(18):2849-2856

  • 相关文献

[1]Molecular characterization of enolase gene from Taenia multiceps. Li, W. H.,Qu, Z. G.,Zhang, N. Z.,Yue, L.,Jia, W. Z.,Luo, J. X.,Yin, H.,Fu, B. Q.,Jia, W. Z.,Luo, J. X.,Yin, H.,Fu, B. Q..

[2]Transcriptomic analysis of the larva Taenia multiceps. Li, W. H.,Zhang, N. Z.,Yang, Y.,Li, L.,Yan, H. B.,Li, T. T.,Qu, Z. G.,Jia, W. Z.,Fu, B. Q.,Yue, L.,Jia, W. Z.,Fu, B. Q.. 2017

[3]Molecular characterization of subcutaneous and muscular coenurosis of goats in United Arab Emirates. Jia, W. Z.,Yan, H. B.,Schuster, R. K.,Varcasia, A.,Manunta, M. L.,Pipia, A. P.,Garippa, G.,Scala, A..

[4]Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1. Li, Wen Hui,Jia, Wan Zhong,Qu, Zi Gang,Xie, Zhi Zhou,Luo, Jian Xun,Yin, Hong,Fu, Bao Quan,Sun, Xiao Lin,Blaga, Radu. 2013

[5]Preliminary Analysis of Taenia multiceps Metacestode Antigens by Two- Dimensional Electrophoresis. Li, Wen Hui,Qu, Zi Gang,Zhang, Nian Zhang,Yue, Long,Gai, Wen Yan,Jia, Wan Zhong,Luo, Jian Xun,Yin, Hong,Fu, Bao Quan,Jia, Wan Zhong,Luo, Jian Xun,Yin, Hong,Fu, Bao Quan. 2014

[6]Genetic variation of main parents of hybrid rice in China was revealed with simple sequence repeat markers. Li, YH,Xiao, H,Zhang, CQ,Hu, GC,Yu, YH,Jia, JZ,Sun, ZX. 1999

[7]Intraspecific and interspecific variation in Setaria revealed by RAPD analysis. Li, Y,Jia, JZ,Wang, Y,Wu, SZ. 1998

[8]Population Genetic Analysis of the Rice Stem Borer, Chilo suppressalis, in the South China. Hou Mao-lin,Liu Yu-di,Wu Yu-chun,Liu Gui-qin. 2013

[9]Population Genetic Analyses of Fusarium asiaticum Populations from Barley Suggest a Recent Shift Favoring 3ADON Producers in Southern China. Zhang, Z.,Chen, W. Q.,Xu, J.,Xu, J. S.,Feng, J.,van der Lee, T.,Waalwijk, C.,Yang, L.,Yu, D.. 2010

[10]Identification of QTLs for seed dormancy in rice (Oryza sativa L.). Xie, Kun,Jiang, Ling,Lu, BingYue,Yang, ChunYan,Li, LinFang,Liu, Xi,Zhang, Long,Zhao, ZhiGang,Wan, JianMin,Wan, JianMin.

[11]Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation. Leng, Xue,Li, Zhenguang,Wang, Fengxue,Wang, Wei,Zhang, Xiuhua,Wu, Hua,Xia, Mingqi,Li, Xiyu,Wu, Hua.

[12]Identification of quantitative trait loci for drought tolerance at seedling stage by screening a large number of introgression lines in maize. Hao, Z.,Liu, X.,Li, X.,Xie, C.,Li, M.,Zhang, D.,Zhang, S.,Hao, Z.,Xu, Y..

[13]Advances in molecular identification, taxonomy, genetic variation and diagnosis of Toxocara spp.. Chen, Jia,Zhou, Dong-Hui,Xu, Min-Jun,Huang, Si-Yang,Li, Ming-Wei,Zhu, Xing-Quan,Chen, Jia,Nisbet, Alasdair J.,Li, Ming-Wei,Wang, Chun-Ren,Zhu, Xing-Quan,Zhu, Xing-Quan.

[14]AFLP analyses of genetic variation of Eupatorium adenophorum (Asteraceae) populations in China. Huang, W. K.,Wan, F. H.,Guo, J. Y.,Peng, D. L.,Huang, W. K.,Gao, B. D.,Xie, B. Y..

[15]Genetic diversity and differentiation of Ankole cattle populations in Uganda inferred from microsatellite data. Kugonza, D. R.,Mpairwe, D.,Kiwuwa, G. H.,Kugonza, D. R.,Jianlin, H.,Okeyo, A. M.,Hanotte, O.,Jianlin, H.,Nabasirye, M..

[16]Genetic variation in V gene of class II Newcastle disease virus. Hao, Huafang,Liu, Peng,Ren, Shanhui,Gao, Xiaolong,Wang, Yanping,Wang, Xinglong,Zhang, Shixia,Yang, Zengqi,Chen, Shengli.

[17]Genetic diversity among a founder parent and widely grown wheat cultivars derived from the same origin based on morphological traits and microsatellite markers. Li, X. J.,Xu, X.,Yang, X. M.,Li, X. Q.,Liu, W. H.,Gao, A. N.,Li, L. H.,Li, X. J.,Xu, X..

[18]Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kuhn. Gao, L.,Chen, W. Q.,Liu, T. G..

[19]Genetic Diversity of Rice False Smut Fungus, Ustilaginoidea virens and its Pronounced Differentiation of Populations in North China. Zhou, Y. -L.,Pan, Y. -J.,Xie, X. -W.,Zhu, L. -H.,Xu, J. -L.,Li, Z. -K.,Li, Z. -K.,Wang, S..

[20]Enhancing genetic gain in the era of molecular breeding. Xu, Yunbi,Zou, Cheng,Xie, Chuanxiao,Xu, Yunbi,Zhang, Xuecai,Li, Ping,Lu, Yanli,Prasanna, Boddupalli M.,Olsen, Michael S..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台