Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth
文献类型: 外文期刊
作者: Yi Deng-xia 1 ; Cui Lei 1 ; Liu Yu-mei 1 ; Zhuang Mu 1 ; Zhang Yang-yong 1 ; Fang Zhi-yuan 1 ; Yang Li-mei 1 ;
作者机构: 1.Chinese Acad Agr Sci, Key Lab Hort Crops Genet Improvement, Minist Agr Inst Vegetables & Flowers, Beijing 100081, Peoples R China
关键词: Bacillus thuringiensis cry1Ba3;cabbage;diamondback moth;resistance
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2011 年 10 卷 11 期
页码:
收录情况: SCI
摘要: To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
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