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Controlled Freezing and Open-Pulled Straw (OPS) Vitrification of In vitro Produced Bovine Blastocysts Following Analysis of ATP Content and Reactive Oxygen Species (ROS) Level

文献类型： 外文期刊

作者： Zhao Xue-ming 1 ; Du Wei-hua 1 ; Wang Dong 1 ; Hao Hai-sheng 1 ; Qin Tong 1 ; Liu Yan 1 ; Zhu Hua-bin 1 ;

作者机构： 1.Chinese Acad Agr Sci, IAS, Embryo Biotechnol & Reprod Lab, Beijing 100193, Peoples R China

关键词： ATP content;controlled freezing;in vitro production blastocysts;OPS vitrification;ROS level

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： To our knowledge, no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT) by controlled freezing and vitrification. This experiment, therefore, was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification. Adenosine-5'-triphosphate (ATP) content and reactive oxygen species (ROS) level in blastocysts were also analyzed. Firstly, for each type of blastocyst (IVF, ICSI or SCNT), significant differences were observed between the survival rates of the controlled freezing ((81.56 +/- 2.33), (68.18 +/- 4.72) or (47.89 +/- 5.83)%) and OPS vitrification groups ((92.24 +/- 4.54), (82.40 +/- 3.76) or (78.71 +/- 5.91)%; P<0.05). Secondly, for each type of blastocyst (IVF, ICSI or SCNT), ATP content was significantly decreased after controlled freezing or vitrification, and the ATP content in the controlled freezing group (0.43 +/- 0.06), (0.35 +/- 0.05) or (0.21 +/- 0.02) pmol) was significantly lower than that found in the OPS vitrification group (0.62 +/- 0.04), (0.46 +/- 0.03) or (0.30 +/- 0.01) pmol; P<0.05). Thirdly, ROS level in fresh IVF ((47.33 +/- 3.56) c.p.s (counted photons per second), ICSI ((36.51 +/- 2.58) c.p.s) or SCNT blastocysts ((26.44 +/- 1.49) c.p.s) was significantly lower than that found in the OPS vitrification group ((72.14 +/- 4.31), (58.89 +/- 3.89) or (40.11 +/- 5.73) c.p.s; P<0.05), but higher than that of the controlled freezing group (34.41 +/- 3.32), (23.13 +/- 1.26) or (15.46 +/- 2.45) c.p.s; P<0.05). The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF, ICSI or SCNT than controlled freezing. Furthermore, both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.

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