A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers
文献类型： 外文期刊
作者： Zhang, Y. P. 1 ; Tan, H. H. 1 ; Cao, S. Y. 2 ; Wang, X. C. 1 ; Yang, G. 1 ; Fang, J. G. 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China
2.Chinese Acad Agr Sci, Zhengzhou Inst Pomol, Zhengzhou, Peoples R China
关键词： Pomegranate;Cultivar identification diagram;Verification;RAPD fingerprints
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2012 年 11 卷 3 期
页码：
收录情况： SCI
摘要： DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plants; a cultivar identification diagram can be manually generated and used as key reference information for quick identification of plant and/or seed samples. Forty-seven pomegranate varieties popularly cultivated in various provinces of China were subjected to RAPD marker analysis. Using the cultivar identification diagram strategy, they were clearly separated by the fingerprints of 11 RAPD primers. The utility and accuracy of the cultivar identification diagram analysis results were confirmed by the identification of three randomly chosen groups of cultivars among the 47 varieties.
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