Purification of Transglutaminase from High Salt Culture by Gel and Cation Exchange Chromatography
文献类型: 外文期刊
作者: Zhang Li-Li 1 ; Zhang Lan-Wei 1 ; Du Ming 1 ; Han Xue 1 ; Yi Hua-Xi 1 ; Zhang Ying-Chun 1 ; Zhang Yan-He 2 ; Ma Wei 1 ;
作者机构: 1.Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China
2.NE Agr Univ, Food Coll, Harbin 150030, Peoples R China; Chinese Acad Agr Sci, Natl Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150001, Peoples R China
关键词: Transglutaminase;Purification;Liquid chromatography/tandem mass spectrometry;Gel chromatography;Cation exchange chromatography
期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )
ISSN: 0253-3820
年卷期: 2012 年 40 卷 8 期
页码:
收录情况: SCI
摘要: To purify the transglutaminase from high-salt medium, gel filtration combined with ion exchange chromatography method was employed. Two-step chromatographic conditions (flow rate and the sample volume) were optimized. The final sample volume and a flow rate for Gel chromatography were 6 mL and 0. 25 mL/min, respectively. Conditions for ion-exchange chromatography were a sample volume of 50 mL and flow rate of 3 mL/min. Enzyme was purified 4. 22-fold, the specific activity and the recovery rate were 17. 33 U/mg protein and 77. 5%, respectrively. A comparison between liquid chromatography/tandem mass spectrometric identification and the protein database was carried out and the results showed that the purified enzyme had high homology with AAN01353.
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