The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana
文献类型: 外文期刊
作者: Jiang Wei-yu 2 ; Geng Li-li 1 ; Dai Ping-li 3 ; Lang Zhi-hong 4 ; Shu Chang-long 1 ; Lin Yi 2 ; Zhou Ting 3 ; Song Fu-pin 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China
3.Chinese Acad Agr Sci, Key Lab Pollinating Insect Biol, Minist Agr, Inst Apicultural Res, Beijing 100093, Peoples R China
4.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 10008
关键词: Bacillus thuringiensis;intestinal bacteria;horizontal gene transfer;transgenic maize;Apis cerana;biosafety
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2013 年 12 卷 3 期
页码:
收录情况: SCI
摘要: Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and Conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL(-1)) and supercoiled plasmid DNA (800 ng mL(-1)) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon's index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.
- 相关文献
作者其他论文 更多>>
-
A New Method for Exonuclease Activity Analysis of Apurinic/Apyrimidinic Endonuclease 1 and Application in Heavy-polluted Ramie
作者:Chen Ping;Gao Gang;Chen Kun-Mei;Zhu Ai-Guo;Zhou Ting;Luo Ru-Xin;Liu Bin
关键词:Apurinic/apyrimidinic endonuclease 1; Exonuclease activity; Antibiotics; Heavy metal ions; Ramie; Phytoremediation
-
Effects of sustained cold and heat stress on energy intake, growth and mitochondrial function of broiler chickens
作者:SU Hong-guang;ZHOU Ting;ZHANG Shao-shuai
关键词:Broiler chicken;Cytochrome c oxidase;Energy intake;Metabolizability;Cold stress;Heat stress
-
Magnetic Solid Phase Extraction Based on Ferroferric Oxide/Nitrogen-doped Graphene for Determination of Six Kinds of Carbonyl Compounds in Water Samples
作者:Cao Xiao-Ji;Zhou Ting;Xu Jia-Hui;Cao Xiao-Ji;Ye Xue-Min;Chai Yun-Feng
关键词:Nitrogen-doped graphene; Magnetic solid-phase extraction; High resolution mass spectrometry; Carbonyl compounds
-
Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916
作者:Xiao Ya-jing;Sun Deng-mei;Xiao Ya-jing;Gao Tan-tan;Peng Qi;Zhang Jie;Song Fu-ping
关键词:methylerythritol phosphate pathway; transcription; Bacillus subtilis; biological control
-
Alanine-substituted mutant on Gly(373) and Asn(375) of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera
作者:Zhou Zi-shan;Liang Ge-mei;Song Fu-ping;Zhang Jie
关键词:Bacillus thuringiensis; Cry1Ai; Domain II-loop2; Helicoverpa armigera; binding affinity
-
High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
作者:Xu Deng;Shu Changlong;Song Fuping;Zhang Jie;Xu Deng;Lu Fan;Zhang Minhong;Feng Jinghai
关键词:broilers;Bt-rice;high-throughput sequencing-based analysis;intestinal microbiota
-
Identification of similar transcriptional regulatory mechanisms in multiple cry genes in Bacillus thuringiensis HD12
作者:Sun Dong-mei;Song Zhi-ru;Peng Qi;Shu Chang-long;Zhang Jie;Song Fu-ping
关键词:Bacillus thuringiensis;transcription;cry genes;insecticide



