Development of SSR Markers for a Phytopathogenic Fungus, Blumeria graminis f.sp tritici, Using a FIASCO Protocol
文献类型: 外文期刊
作者: Wang Meng 2 ; Xue Fei 4 ; Yang Peng 1 ; Duan Xia-yu 1 ; Zhou Yi-lin 1 ; Shen Chong-yao 2 ; Zhang Guo-zhen 2 ; Wang Bao-to 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100093, Peoples R China
2.China Agr Univ, Minist Agr, Dept Plant Pathol, Key Lab Plant Pathol, Beijing 100193, Peoples R China
3.Huaian Entry Exit Inspect & Quarantine Bur, Huaian 223001, Jiangsu, Peoples R China
4.Shihezi Univ, Coll Agron, Shihezi 832000, Peoples R China
5.Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Peoples R China
关键词: Blumeria graminis f.sp tritici;microsatellite;SSR;FIASCO
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2014 年 13 卷 1 期
页码:
收录情况: SCI
摘要: Simple sequence repeats (SSR) have been widely used as molecular markers due to their abundance and high polymorphism. However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis f.sp. tritici. From (AC)(10) and (AG)(10) enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO (fast isolation by AFLP of sequences containing repeats) protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces (cities) in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 (mean 0.025) and expected heterozygosity ranged from 0.297-0.816 (mean 0.538). These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping of Bgt. SSR markers of Bgt need to be further explored.
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