Penetration of a Single Domain of Bacillus thuringiensis Cry1Ie-Domain I to a Lipid Membrane In vitro
文献类型: 外文期刊
作者: Guo Shu-yuan 1 ; Li Jie 1 ; Chen Zhen 1 ; He Kang-lai 2 ;
作者机构: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
关键词: Bacillus thuringiensis;Cry1Ie;domain I of Cry protein;monolayer insertion
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2014 年 13 卷 5 期
页码:
收录情况: SCI
摘要: Domain I of the activated Crystal protein from Bacillus thuringiensis has a seven alpha-helix bundle structure, which is responsible for membrane channel formation in its insecticidal mechanism. Cry1Ie is toxic to Asian corn borer, Ostrinia furnacalis (Guenee), and plays important roles in insect biological control. The domain I from Cry1Ie has been expressed and purified in its normal conformation, as embedded in the full length homologous toxin structure. The membrane insertion ability of this single domain was compared with the full length homologous toxin using a monolayer insertion experiment. The results indicated that the Cry1Ie-domain I had the ability to insert into the lipid monolayer, and this ability is greater than that of the IE648 toxin. However, the state of insertion is not stable and remains for only a short period of time. The Cry1Ie-domain I plays no role in receptor binding as it had a nonspecific binding with the brush border membrane vesicles of the Asian corn borer.
- 相关文献
作者其他论文 更多>>
-
Developing transgenic maize (Zea mays L.) with insect resistance and glyphosate tolerance by fusion gene transformation
作者:Lang Zhi-hong;Lu Wei;Zhu Li;Lin Min;Huang Da-fang;Zhang Jie;He Kang-lai
关键词:LP4/2A;gene stacking;transgenic maize;insect resistance;glyphosate tolerance
-
轻小型喷灌机组的运行速率与水头损失的研究
作者:CAO Hua;曹华;GUO Fengtai;郭凤台;FAN Yongshen;范永申;DUAN Fuyi;段福义;Chen Zhen;陈震
关键词:喷灌机组;运行速率;水头损失;进口压力
-
Acquisition of Insect-Resistant Transgenic Maize Harboring a Truncated cry1Ah Gene via Agrobacterium-Mediated Transformation
作者:Huang Da-fang;Li Xiu-ying;Lang Zhi-hong;Zhu Li;Huang Da-fang;Zhang Jie;He Kang-lai
关键词:Bacillus thuringiensis;truncated cry1Ah gene;insectresistant maize;stable inheritance
-
Molecular Taxonomy of Conogethes punctiferalis and Conogethes pinicolalis (Lepidoptera: Crambidae) Based on Mitochondrial DNA Sequences
作者:Zhang Tian-tao;Wang Zhen-ying;He Kang-lai;Li Jing;Wang Jing;Liu Yong
关键词:yellow peach moth;Conogethes punctiferalis;Conogethes pinicolalis;mitochondrial DNA sequence
-
人民胜利渠灌区不同典型年灌溉供水能力研究
作者:CHEN Zhen;陈震;LI Jin-shan
关键词:灌溉管理;作物需水;供水能力;水资源利用率
-
Wolbachia Infection in Populations of Ostrinia furnacalis: Diversity, Prevalence, Phylogeny and Evidence for Horizontal Transmission
作者:Li Jing;Wang Zhen-ying;He Kang-lai;Bourguet, Denis
关键词:Wolbachia;Ostrinia furnacalis;infection diversity;prevalence pattern;horizontal transmission
-
Olfactory Response of Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) to Volatiles Emitted by Mungbean Plants
作者:Wang Zhen-ying;He Kang-lai;Dae-joon, Im
关键词:Trichogramma ostriniae;olfactory response;Vigna radiata;plant volatile



