您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The 14-3-3 gene AaGRF1 positively regulates cold tolerance in kiwifruit

文献类型： 外文期刊

作者： Zhang, Zhenzhen 1 ; Li, Sumei 1 ; Sun, Shihang 1 ; Li, Hongli 3 ; Zhang, Qina 1 ; Li, Yihang 1 ; Li, Yukuo 1 ; Liu, Mingyu 1 ; Li, Congcong 1 ; Sun, Leiming 1 ; Lin, Miaomiao 1 ; Qi, Xiujuan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Zhengzhou 450009, Peoples R China

2.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang 453500, Peoples R China

3.Heilongjiang Acad Forestry Sci, Mudanjiang Branch, Mudanjiang 157000, Peoples R China

关键词： Kiwifruit; AaGRF1; Cold tolerance; RNA-seq

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2025 年 353 卷

页码：

收录情况： SCI

摘要： Low temperatures severely threaten the growth and development of kiwifruit. Research has demonstrated that proteins belonging to the 14-3-3 family play a pivotal regulatory function in the ability of plants to resist stress. However, this specific roles of the genes in kiwifruit cold tolerance remain unclear. It had been identified that beta-amylase gene, AaBAM3.1, exhibits a positive regulatory effect on kiwifruit's tolerance to low temperature. In our research, we obtained the Actinidia arguta 14-3-3 gene general regulatory factor 1 (AaGRF1) from yeast one- hybrid (Y1H) screening library of the AaBAM3.1 promoter; the expression level of AaGRF1 was enhanced by low- temperature stress. Subcellular localization, Y1H and dual-LUC assay indicated that the AaGRF1 protein resides within the nucleus and possesses the ability to interact with the AaBAM3.1 promoter. Moreover, we also studied the role of AaGRF1 gene in cold resistance of kiwifruit. When AaGRF1 was overexpressed in kiwifruit, the transgenic plants exhibited enhanced cold tolerance. The level of antioxidants and soluble sugars in these plants were elevated compared to wild-type (WT) lines. RNA-seq of the transgenic and WT lines revealed that AaGRF1 might interact with genes in the 'ascorbate-glutathione' and 'starch and sucrose' pathways, thereby enhancing the cold resistance of kiwifruit. In summary, we hypothesize that the 14-3-3 gene AaGRF1 may positively modulate the cold resistance in kiwifruit by accumulating more antioxidants and soluble sugars.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台