Unveiling the development of cotton somatic embryos: Integrating spatial transcriptomics and metabolomics
文献类型： 外文期刊
作者： Qin, Aizhi 1 ; Zhou, Yaping 1 ; Yu, Xiaole 1 ; Ge, Xiaoyang 2 ; Liu, Zhixin 1 ; Liu, Hao 1 ; Tran, Lam-Son Phan 3 ; Sun, Xuwu 1 ;
作者机构： 1.Henan Univ, Sch Life Sci, State Key Lab Cotton Biobreeding & Integrated Util, Key Lab Plant Stress Biol, 85 Minglun St, Kaifeng 475001, Peoples R China
2.Chinese Acad Agr Sci, Inst Cotton Res, Anyang 455000, Peoples R China
3.Texas Tech Univ, Inst Genom Crop Abiot Stress Tolerance, Dept Plant & Soil Sci, Lubbock, TX 79409 USA
关键词： Cotton; Spatial transcriptome; Spatial metabolome; Somatic embryo
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）
ISSN： 0926-6690
年卷期： 2025 年 230 卷
页码：
收录情况： SCI
摘要： Plant development necessitates a diverse array of cell types, metabolites, and intricate gene regulation patterns. Consequently, investigating the spatial localization of cells within tissues, the spatial expression of genes, and the distribution of both primary and secondary metabolites in plants is crucial. The process of somatic embryo development is vital for the genetic enhancement of cotton, yet it remains poorly understood. Moreover, there is currently a scarcity of available data resources on the spatial expression patterns of genes and metabolites in cotton somatic embryo cells. In this study, we employed 10 x Visium spatial transcriptomics (ST) technology and air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) spatial metabolomics (SM) technology to establish sequencing libraries and metabolomes of upland cotton (Gossypium hirsutum) somatic embryos, specifically focusing on globular, torpedo, and cotyledonary embryos. Specifically, we have highlighted the spatially regulated gene networks and metabolite gradients critical to embryogenesis. For instance, the integrated analysis of ST and SM revealed that polyamines (putrescine, spermidine, and spermine) and their biosynthesis-related genes (ADC, ODC, ARGAH1, SPDSYN1, and SPMS) were highly expressed in globular embryos. These findings suggest that polyamines and their biosynthesis-related genes may play a pivotal role in the conversion of embryogenic callus into somatic embryos in cotton. In summary, we propose an integrated spatially resolved multiomics approach, which will aid in deciphering the complex regulatory networks involved in the development of cotton somatic embryos.
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study revealed candidate genes associated with leaf size in alfalfa (Medicago sativa L.)
作者：Xu, Ming;Xu, Yanchao;Liu, Hao;Yang, Qingchuan;Long, Ruicai;Chen, Lin;He, Fei;Liu, Qingsong
关键词：Alfalfa; GWAS; Leaf size; Haplotype analysis
-
Effects of Chicken Manure Combinations on Compost Maturity and Microbial Community Dynamics in Industrial-Scale Composting
作者：Wang, Weiwei;Zhang, Peng;He, Huiban;Liu, Hao;He, Haoxing;Cui, Zongjun;Yuan, Xufeng;Yuan, Xufeng;Wen, Boting;Yan, Jing
关键词：Aerobic composting; Chicken manure; Compost maturity; Microbial community
-
Climatic seasonality shapes insect community composition on the Mongolian Plateau
作者：Tang, Kuanyan;Hilal, Mian Gul;Yue, Hongru;Ding, Xiaolong;Xing, Yifang;Zhao, Jinming;Liu, Yang;Lin, Kejian;Wang, Ning;Liu, Hao;He, Zilong
关键词：Climatic seasonality; Steppe; Biodiversity; Insect community composition; PLS-PM model
-
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
-
Comprehensive analysis of epigenetic modifications in alfalfa under cadmium stress
作者：Chen, Lin;Li, Xianyang;Liu, Hao;He, Fei;Li, Mingna;Long, Ruicai;Wang, Xue;Kang, Junmei;Yang, Qingchuan
关键词：Cadmium stress; DNA methylation; N 6 methylation; Gene expression; Alfalfa
-
Remodelling autoactive NLRs for broad-spectrum immunity in plants
作者：Wang, Junzhu;Chen, Tianyuan;Zhang, Zhendong;Song, Mengjie;Shen, Tianxin;Wang, Xin;Zheng, Xiyin;Wang, Yan;Song, Ke;Qi, Tiancong;Liu, Yule;Wang, Junzhu;Chen, Tianyuan;Zhang, Zhendong;Song, Mengjie;Shen, Tianxin;Wang, Xin;Zheng, Xiyin;Wang, Yan;Song, Ke;Qi, Tiancong;Liu, Yule;Wang, Junzhu;Chen, Tianyuan;Zhang, Zhendong;Song, Mengjie;Shen, Tianxin;Wang, Xin;Zheng, Xiyin;Wang, Yan;Song, Ke;Qi, Tiancong;Liu, Yule;Ge, Xiaoyang;Li, Fuguang;Xu, Kai;Hong, Yiguo;Hong, Yiguo;Hong, Yiguo
关键词：
-
Spatiotemporal transcriptome and metabolome landscapes of cotton fiber during initiation and early development
作者：Sun, Xuwu;Wang, Xingxing;Ge, Xiaoyang;Zhang, Xianliang;Hu, Guanjing;Li, Wei;Zang, Xinshan;Dai, Shuai;Ma, Xiongfeng;Sun, Xuwu;Qin, Aizhi;Liu, Zhixin;Guo, Chenxi;Yu, Xiaole;Yang, Jincheng;Zhou, Yaping;Liu, Yumeng;Hu, Mengke;Liu, Hao;Zhao, Zihao;Sun, Susu;Wang, Xingxing;Ge, Xiaoyang;Zhang, Xianliang;Hu, Guanjing;Li, Wei;Zang, Xinshan;Dai, Shuai;Ma, Xiongfeng;Lu, Yao;He, Jiuming;Yong-Villalobos, Lenin;Herrera-Estrella, Luis;Tran, Lam-Son Phan
关键词：
