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Three Heat Shock Protein Genes from Bactrocera (Tetradacus) minax Enderlein: Gene Cloning, Characterization, and Association with Diapause

文献类型： 外文期刊

作者： Lu, Z. C. 1 ; Wang, L. H. 1 ; Zhang, G. F. 1 ; Wan, F. H. 1 ; Guo, J. Y. 1 ; Yu, H. 3 ; Wang, J. B. 3 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100081, Peoples R China

2.Minist Agr, Ctr Management Invas Alien Species, Beijing, Peoples R China

3.Henan Inst Sci & Technol, Dept Entomol, Xinxiang, Henan Province, Peoples R China

关键词： Diapause;gene characterization;gene expression profile;heat shock protein

期刊名称：NEOTROPICAL ENTOMOLOGY （ 影响因子：1.434； 五年影响因子：1.46 ）

ISSN： 1519-566X

年卷期： 2014 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Bactrocera (Tetradacus) minax Enderlein is a major pest to wild and cultivated species of citrus. Bactrocera minax produces one generation per year with a long pupal diapause period of over 6 months, which hinders efforts to obtain vast numbers of insects under standard room conditions. Determining the mechanisms of diapause is significantly important for obtaining large quantities of these insects. To characterize the heat shock protein (Hsp) genes of B. minax and to unravel their potential contribution to diapause, we performed 3' and 5' RACE to isolate the complementary DNA (cDNA) sequences, bioinformatics to examine the phylogenetic relationships, and real-time quantitative PCR to detect the expression patterns of three Hsp genes during various developmental stages. These results represent the first characterization of the three Hsp genes of B. minax; the open reading frames of Bmhsp23, Bmhsp70, and Bmhsp90 were 510, 1,911, and 1,089 bp, encoding 170, 636, and 363 amino acids, respectively. BmHsp70 and BmHsp90 displayed high identity to previously identified Hsp70 and Hsp90 genes, respectively. BmHsp23 displayed varying similarity, from 28 to 83%, to previously identified small Hsps. Bmhsp23 messenger RNA (mRNA) expression was found to be upregulated during diapause initiation, maintenance, and termination. Bmhsp70 mRNA expression peaked during diapause initiation. Bmhsp90 mRNA expression remained at a relatively low level during deep diapause. Our present results suggest that Bmhsp70 might play an important role in diapause initiation, while Bmhsp23 in diapause initiation and maintenance and Bmhsp90 in diapause regulation. These results improve our understanding of the mechanism of diapause in B. minax at the molecular level.

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