您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The homeobox gene Distal-less is critical for the development of adult body appendages in Bombyx mori

文献类型： 外文期刊

作者： Man, Guan 1 ; Guo, Peilin 1 ; Liu, Yutong 1 ; Kang, Hongxia 1 ; Chen, Kai 1 ; Yu, Ye 1 ; Tan, Anjiang 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang, Peoples R China

关键词： appendage development; Bombyx mori; CRISPR/Cas9; Distal-less

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.3； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2025 年

页码：

收录情况： SCI

摘要： The development of adult body appendages is critical for insects. The homeobox gene Distal-less (Dll) is known for its role in proximodistal patterning, and its role in the development of the appendage has been well studied in the model organism Drosophila melanogaster. However, the biological function of Dll in other insects still remains to be further verified. In this study, we investigated the biological function of Dll in the lepidoptera model insect Bombyx mori. Similar to the expression patterns observed in D. melanogaster, BmDll was highly expressed in the tibia and tarsus of the legs. The BmDll mutants obtained through the binary transgenic CRISPR/Cas9 system appeared to thrive normally and no apparent deleterious phenotypes were observed throughout the embryonic and larval stages. Nevertheless, mutants exhibited severe defects in the development of the appendage, especially at the distal end of legs, from pupal stage to adult stage. RNA-seq analysis revealed that metabolism pathways, extracellular matrix (ECM)-receptor interaction and structural constituent of the cuticle were dysregulated in mutant silkworms. Our study provided genetic evidence that Dll plays an important role in the development of B. mori adult body appendages.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台