中国农业科学院机构知识库

Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

文献类型：外文期刊

作者： Zhang Yong ¹ ; Fan Jia ¹ ; Sun Jing-rui ¹ ; Chen Ju-lian ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Schizaphis graminum;salivary protein C002;cDNA clone;siRNA

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （影响因子：2.848；五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： The full-length cDNA of functionally-unknown salivary protein 0002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.

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