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Molecular identity of ramie germplasms using simple sequence repeat markers

文献类型： 外文期刊

作者： Luan, M. B. 1 ; Chen, B. F. 2 ; Zou, Z. Z. 3 ; Zhu, J. J. 1 ; Wang, X. F. 1 ; Xu, Y. 1 ; Sun, Z. M. 1 ; Chen, J. H. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Stem Fiber Biomass & Engn Microbiol, Minist Agr, Inst Bast Fiber Crops, Changsha, Hunan, Peoples R China

2.North Sichuan Med Coll, Dept Med Biol, Nanchong, Sichuan, Peoples R China

3.Yiyang Med Sch, Yiyang, Peoples R China

关键词： Molecular identity;Ramie;Simple sequence repeat

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： DNA identity is highly effective and efficient for distinguishing crop varieties regardless of their phenotypic similarities. To establish DNA identity in ramie, 21 simple sequence repeat primers were amplified in 108 accessions of domestic and exotic ramie germplasms. Sixty polymorphic bands were obtained, with an average of 2.9 bands per locus and 2-8 band types per primer locus (average of 5.19 band types). The Simpson's diversity index of the 21 simple sequence repeat loci ranged from 0.158 to 0.808 with an average of 0.612. There was large difference in the specific index in the germplasm tested, from 44.082 to 218.163, with an average of 83.620. Based on allele band type, 8 primer pairs were selected for DNA fingerprinting of the 108 genotypes. The combination of the 8 primer pairs were found to be very effective for distinguishing these genotypes, indicating that they can be used in the molecular DNA identity of ramie.

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