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Heterozygosities and genetic relationship of tea cultivars revealed by simple sequence repeat markers and implications for breeding and genetic mapping programs

文献类型： 外文期刊

作者： Tan, L. Q. 1 ; Zhang, C. C. 2 ; Qi, G. N. 1 ; Wang, L. Y. 2 ; Wei, K. 2 ; Chen, S. X. 1 ; Zou, Y. 1 ; Wu, L. Y. 2 ; Cheng, H 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Hort, Tea Sci Dept, Yaan, Sichuan, Peoples R China

2.Chinese Acad Agr Sci, Tea Res Inst, Natl Ctr Tea Improvement, Hangzhou, Zhejiang, Peoples R China

3.Minist Agr, Key Lab Tea Plant Biol & Resources Utilizat, Hangzhou, Zhejiang, Peoples R China

4.Minist Agr, Key Lab Tea Plant Biol & Reso

关键词： Tea cultivars;Heterozygosity;Genetic mapping;Simple sequence repeat marker

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Genetic maps are essential tools for quantitative trait locus analysis and marker-assisted selection breeding. In order to select parents that are highly heterozygous for genetic mapping, the heterozygosity (H-S) of 24 tea cultivars (Camellia sinensis) was analyzed with 72 simple sequence repeat markers. In total, 359 alleles were obtained with an average of 4.99 per marker. The H-S varied greatly from 37.5 to 71.0% with an average of 51.3%. On average, tea cultivars from Fujian Province showed a higher level of heterozygosity (59.8%) than those from Zhejiang (48.5%) and Yunnan (44.5%), and the 12 national tea cultivars were generally more heterozygous than the 12 provincial cultivars. Unweighted pair-group analysis using the arithmetic average grouping divided the 24 cultivars into 2 groups that are consistent with the morphological classification. All dual combinations of the 24 cultivars were studied to calculate the percentage of mappable markers when using pseudo-testcross mapping strategy, and results showed that this value also varied greatly from 51.4 to 90.3%. The genetic relationships and H-S differences among different cultivars were discussed, and tea cultivars with high H-S were recommended as cross parents for genetic mapping programs.

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