Interspecific Hybridization between Actinidia arguta and A. deliciosa and Embryo Culture In Vitro of Its F-1 Hybrids
文献类型： 外文期刊
作者： Qin, H. Y. 1 ; Ai, J. 1 ; Xu, P. L. 1 ; Liu, Y. X. 1 ; Zhao, Y. 1 ; Wang, Z. X. 1 ; Yang, Y. M. 1 ; Fan, S. T. 1 ; Li, X. Y.;
作者机构： 1.Chinese Acad Agr, Lab Special Wild Plant Physiol, Inst Special Wild Econ Anim & Plant Sci, Zuojia Town, Jilin, Peoples R China
关键词： interspecific hybridization;embryo culture
期刊名称：VIII INTERNATIONAL SYMPOSIUM ON KIWIFRUIT
ISSN： 0567-7572
年卷期： 2015 年 1096 卷
页码：
收录情况： SCI
摘要： Interspecific hybidization was achieved beween Actinidia arguta (4x) females and A. deliciosa (6x) males, and hybrid embryos were cultured on a solidified Murashige and Skoog (MS) basal medium, supplemented with 16 different combinations of 3-indoleacetic acid (IAA), 6-benzylaminopurine (BAP) and gibberellins (GA). Best results were obtained when the F-1 embryos were cultured with supplementation with 1-naphthaleneacetic acid NAA (0.3 mg L-1), BAP (2 mg L-1) and GA (0.2 mg L-1). When the plantlets were cultured in medium with BAP (4 mg L-1) and NAA (0.01 mg L-1) significant improvement in proliferation was achieved within one month. Plantlets were rooted on 1/2 MS medium in combination with different concentrations of indole-3-butyric acid (IBA) or NAA, and high rooting and survival was achieved using 1/2 MS media with NAA (0.2 mg L-1). This study offered a new path for Actinidia breeding.
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