您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli

文献类型： 外文期刊

作者： Khan, Muhammad Moman 1 ; Ali, Aamir 1 ; Kolenda, Rafal 3 ; Olowe, Olugbenga Adekunle 1 ; Weinreich, Joerg 1 ; Li, Ganwu 6 ; Schierack, Peter 1 ;

作者机构： 1.Brandenburg Tech Univ Cottbus, Inst Biotechnol, Univ Pl 1, D-01968 Senftenberg, Germany

2.Pakistan Inst Engn & Appl Sci NIBGE C PIEAS, Natl Inst Biotechnol & Genet Engn Coll, Jhang Rd,POB 577, Faisalabad, Pakistan

3.Wroclaw Univ Environm & Life Sci, Fac Vet Med, Dept Biochem & Mol Biol, Wroclaw, Poland

4.Quadram Inst, Norwich Res Pk, Norwich, England

5.Ladoke Akintola Univ Technol, Coll Hlth Sci, Dept Med Microbiol & Parasitol, Ogbomosho, Oyo, Nigeria

6.Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA

7.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin, Peoples R China

关键词： APEC; Biofilm; Gene complementation; Transposon mutant; VideoScan

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN：

年卷期： 2023 年 19 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundInfections caused by avian pathogenic Escherichia coli (APEC) result in significant economic losses in poultry industry. APEC strains are known to form biofilms in various conditions allowing them to thrive even under harsh and nutrient-deficient conditions on different surfaces, and this ability enables them to evade chemical and biological eradication methods. Despite knowing the whole genome sequences of various APEC isolates, little has been reported regarding their biofilm-associated genes. A random transposon mutant library of the wild-type APEC IMT 5155 comprising 1,300 mutants was analyzed for biofilm formation under nutrient deprived conditions using Videoscan technology coupled with fluorescence microscopy. Seven transposon mutants were found to have reproducibly and significantly altered biofilm formation and their mutated genes were identified by arbitrary PCR and DNA sequencing. The intact genes were acquired from the wild-type strain, cloned in pACYC177 plasmid and transformed into the respective altered biofilm forming transposon mutants, and the biofilm formation was checked in comparison to the wild type and mutant strains under the same conditions.ResultsIn this study, we report seven genes i.e., nhaA, fdeC, yjhB, lysU, ecpR, AJB35136 and fdtA of APEC with significant contribution to biofilm formation. Reintroduction of AJB35136 and fdtA, reversed the altered phenotype proving that a significant role being played by these two O-antigen related genes in APEC biofilm formation. Presence of these seven genes across nonpathogenic E. coli and APEC genomes was also analyzed showing that they are more prevalent in the latter.ConclusionsThe study has elucidated the role of these genes in APEC biofilm formation and compared them to adhesion expanding the knowledge and understanding of the economically significant pathogens.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台