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Modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection

文献类型： 外文期刊

作者： Gao Xue 1 ; Du Xin-hua 1 ; Zhang Lu-pei 1 ; Chen Yan 1 ; Gao Hui-jiang 1 ; Xu Shang-zhong 1 ; Li Jun-ya 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr, Key Lab Farm Anim Genet Resources & Utilizat, Beijing 100193, Peoples R China

2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

关键词： beef cattle;codon bias;modified Bfat-1;hydrodynamic tail vein injection;mice

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Omega-3 polyunsaturated fatty acids (omega-3 PUFAs) are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce omega-3 PUFAs, due to the lack of the omega-3 fatty acid desaturase gene (fat-1 gene). Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias of Bfat-1 gene was increased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the omega-6/omega-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver (P<0.05), and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection (P<0.05). This confirms that the Bfat-1 gene modification was successful, and the protein encoded. was able to catalyze the conversion of omega-6 PUFAs to omega-3 PUFAs.

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