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The Protein Elicitor PevD1 Enhances Resistance to Pathogens and Promotes Growth in Arabidopsis

文献类型: 外文期刊

作者: Liu, Mengjie 1 ; Khan, Najeeb Ullah 1 ; Wang, Ningbo 1 ; Yang, Xiufen 1 ; Qiu, Dewen 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100081, Peoples R China

关键词: PevD1;transgene;disease resistance;growth phenotype;transcriptomics;signaling pathway

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES ( 影响因子:6.58; 五年影响因子:6.478 )

ISSN: 1449-2288

年卷期: 2016 年 12 卷 8 期

页码:

收录情况: SCI

摘要: The protein elicitor PevD1, isolated from Verticillium dahlia, could enhance resistance to TMV in tobacco and Verticillium wilt in cotton. Here, the pevd1 gene was over-expressed in wild type (WT) Arabidopsis, and its biological functions were investigated. Our results showed that the transgenic lines were more resistant to Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 than the WT line was. In transgenic plants, both the germination time and bolting time required were significantly shorter and fresh weights and plant heights were significantly higher than those in the WT line. A transcriptomics study using digital gene expression profiling (DGE) was performed in transgenic and WT Arabidopsis. One hundred and thirty-six differentially expressed genes were identified. In transgenic Arabidopsis, three critical regulators of JA biosynthesis were up-regulated and JA levels were slightly increased. Three important repressors of the ABA-responsive pathway were up-regulated, indicating that ABA signal transduction may be suppressed. One CML and two WRKY TFs involved in Ca2+-responsive pathways were up-regulated, indicating that this pathway may have been triggered. In conclusion, we show that PevD1 is involved in regulating several plant endogenous signal transduction pathways and regulatory networks to enhance resistance and promote growth and development in Arabidopsis.

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